PMID- 7523335
OWN - NLM
STAT- MEDLINE
DCOM- 19941104
LR  - 20190830
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 101
IP  - 4
DP  - 1994 Apr
TI  - Immunohistochemical studies of neurochemical markers in normal human buccal 
      mucosa.
PG  - 235-44
AB  - The content of various substances, such as regulatory peptides, hormones and 
      structural proteins, was investigated in normal buccal mucosa using indirect 
      immunofluorescence. Thin nerve fibres, which from a morphological point of view 
      were most probably sensory, showed immunoreactivity for substance P (SP), 
      calcitonin gene-related peptide (CGRP), neuropeptide K (NPK) and neurokinin A 
      (NKA). Also galanin (GAL), gamma-melanocyte stimulating hormone (gamma-MSH) and 
      somatostatin (SOM) stained thin fibres were found in the propria, which were, 
      however, few in number and the gamma-MSH staining was weak. CGRP, vasoactive 
      intestinal polypeptide (VIP), peptide histidine isoleucine amide (PHI) and 
      neuropeptide Y (NPY) immunoreactive nerve fibres were observed in close 
      connection to blood vessels. SOM positive cells with processes were found, mostly 
      scattered, in the connective tissue. A population of cells within the epithelium 
      also showed somatostatin immunoreactivity. Protein S-100 (S-100) stained distinct 
      populations of cells at two separate locations. In the propria, cells with one or 
      two slender processes were seen, being mostly single but sometimes forming 
      groups. In the epithelium, dendritic cells with many processes with or without 
      'spines' were observed, mainly located to the basal layer of the lamina 
      epithelialis. Single nerve fibres and nerve bundles were also stained. 
      Neurofilament (NF) positive fibres, singly and in bundles, as well as 
      endorgan-like structures were seen. Neuron-specific enolase (NSE) and protein 
      gene product 9.5 (PGP 9.5) both stained the same structures, namely single 
      fibres, nerve bundles, nerves surrounding vessels and innervating muscles and 
      glands (if present in the section), as well as Merkel cells. Also with these two 
      markers endorgan-like structures were seen. No clear innervation of the 
      epithelium could be observed with the markers used. No methionine-enkephalin 
      (ENK) or synaptophysin (SYN) immunoreactive material was found.
FAU - Hilliges, M
AU  - Hilliges M
AD  - Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
FAU - Hellman, M
AU  - Hellman M
FAU - Ahlstrom, U
AU  - Ahlstrom U
FAU - Johansson, O
AU  - Johansson O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 0 (Biomarkers)
RN  - 0 (Neuropeptides)
RN  - 33507-63-0 (Substance P)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 9002-79-3 (Melanocyte-Stimulating Hormones)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers/analysis
MH  - Calcitonin Gene-Related Peptide/analysis
MH  - Cheek
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Melanocyte-Stimulating Hormones/analysis
MH  - Middle Aged
MH  - Mouth Mucosa/*chemistry/*innervation
MH  - Nerve Tissue/chemistry
MH  - Neuropeptides/*analysis
MH  - Substance P/analysis
MH  - Vasoactive Intestinal Peptide/analysis
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1007/BF00315910 [doi]
PST - ppublish
SO  - Histochemistry. 1994 Apr;101(4):235-44. doi: 10.1007/BF00315910.