PMID- 7523428
OWN - NLM
STAT- MEDLINE
DCOM- 19941121
LR  - 20131121
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 14
IP  - 6
DP  - 1994 Nov
TI  - Different pial arteriolar responses to acetylcholine in the newborn and juvenile 
      pig.
PG  - 1088-95
AB  - Using the closed cranial window technique, the present study was designed to test 
      the hypothesis that the pial arteriolar response to acetylcholine is age 
      dependent. In newborn pigs (1-5 days old) pretreated with the phosphodiesterase 
      inhibitor isobutyl methyl xanthine (IBMX), acetylcholine (10(-5) M) produced pial 
      arteriolar constriction with no change in CSF cyclic GMP (cGMP) that was blocked 
      by indomethacin (5 mg/kg i.v.). In contrast, in indomethacin- and IBMX-treated 
      juvenile pigs (3-4 weeks old), acetylcholine (10(-) M) increased the pial 
      arteriolar diameter by 17 +/- 1% and increased CSF cGMP by 2.1 +/- 0.3-fold. 
      Similar vascular and biochemical changes for acetylcholine were observed in 
      juvenile pigs pretreated with only IBMX. In the absence of IBMX, acetylcholine 
      produced modest pial constriction in juvenile pigs. In the IBMX-pretreated 
      juvenile pigs, L-nitroarginine (LNA; 10(-6) M) decreased pial arteriolar diameter 
      by 15 +/- 2% and blocked acetylcholine-induced dilation and associated changes in 
      CSF cGMP. A23187, a calcium ionophore, and sodium nitroprusside (SNP) elicited 
      similar dilation and changes in CSF cGMP in both age groups. LNA blocked A23187 
      dilation, but SNP dilation was unchanged. L-Arginine (10(-3) M) partially 
      restored acetylcholine- and A23187-induced dilation to indomethacin- and 
      LNA-pretreated juvenile pigs. These data show that acetylcholine produces 
      dilation in the juvenile pig through the production of the putative 
      endothelium-derived relaxing factor (EDRF) nitric oxide but does not do so in the 
      new born period. We speculate that contributions of EDRF to the 
      acetylcholine-induced changes in pial arteriolar diameter develop with age.
FAU - Armstead, W M
AU  - Armstead WM
AD  - Department of Physiology, University of Tennessee, Memphis.
FAU - Zuckerman, S L
AU  - Zuckerman SL
FAU - Shibata, M
AU  - Shibata M
FAU - Parfenova, H
AU  - Parfenova H
FAU - Leffler, C W
AU  - Leffler CW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 169D1260KM (Nitroprusside)
RN  - 2149-70-4 (Nitroarginine)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 94ZLA3W45F (Arginine)
RN  - H2D2X058MU (Cyclic GMP)
RN  - N9YNS0M02X (Acetylcholine)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Acetylcholine/*pharmacology
MH  - Aging/*physiology
MH  - Animals
MH  - Animals, Newborn/growth & development/*physiology
MH  - Arginine/analogs & derivatives/pharmacology
MH  - Arterioles/drug effects
MH  - Calcimycin/pharmacology
MH  - Cerebrovascular Circulation/drug effects
MH  - Cyclic GMP/cerebrospinal fluid
MH  - Female
MH  - Male
MH  - Nitroarginine
MH  - Nitroprusside/pharmacology
MH  - Pia Mater/*blood supply
MH  - Swine
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1038/jcbfm.1994.142 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 1994 Nov;14(6):1088-95. doi: 10.1038/jcbfm.1994.142.