PMID- 7523434
OWN - NLM
STAT- MEDLINE
DCOM- 19941024
LR  - 20190920
IS  - 0271-9142 (Print)
IS  - 0271-9142 (Linking)
VI  - 14
IP  - 3
DP  - 1994 May
TI  - Modulation of immunoglobulin production and cytokine mRNA expression in 
      peripheral blood mononuclear cells by intravenous immunoglobulin.
PG  - 178-89
AB  - Intravenous immunoglobulin (IVIG) has the potential to regulate Ig production, 
      but the mechanism(s) responsible for this effect is unknown. In experiments 
      reported here, we examined the ability of IVIG to regulate Ig production in human 
      peripheral blood mononuclear cells (PBMCs) stimulated with pokeweed mitogen 
      (PWM). IVIG (2-10 mg/ml) showed a potent (80-85%) inhibition of PWM-stimulated 
      IgG, IgM, and IgA production. To determine more precisely how IVIG mediated the 
      inhibition of Ig production, we studied Ig promoting cytokine gene expression 
      after PWM stimulation with or without IVIG (2 and 10 mg/ml) using dot-blot 
      techniques. RNA was isolated from PBMCs at predetermined time points and probed 
      with cDNAs specific for human cytokines (IL-1 beta, IL-2, IL-2R, IL-4, IL-5, 
      IL-6, gamma-IFN, and TNF-alpha). IL-6 mRNA accumulation was maximal at 4.5 hr 
      post-PWM stimulation and was inhibited 64-75% when IVIG (10 mg/ml) was present. 
      gamma-IFN mRNA levels peaked at 72 hr poststimulation and were also 68-75% 
      inhibited by IVIG. IL-2 mRNA levels peaked at 4.5 hr and were 23-46% inhibited by 
      IVIG. The inhibitory effect of IVIG on production of these cytokines (IL-6 and 
      gamma-IFN) was also observed at the protein level in sonicated PBMCs after 
      incubation with PWM and IVIG. The mRNA levels for other cytokines were not or 
      only minimally inhibited by IVIG. Addition of IL-6, gamma-IFN, or IL-2 partially 
      restored Ig production in IVIG-treated PWM-stimulated cultures, suggesting that 
      inhibition of other cytokines or another mechanism(s) independent of cytokine 
      inhibition might also be involved, although inhibition of IL-6, gamma-IFN, and 
      IL-2 may be one of the critical factors in the suppression of Ig production by 
      IVIG.
FAU - Toyoda, M
AU  - Toyoda M
AD  - Ahmanson Pediatrics Center, Steven Spielberg Pediatric Research Laboratory, 
      Cedars-Sinai Medical Center/University of California, Los Angeles School of 
      Medicine 90048.
FAU - Zhang, X
AU  - Zhang X
FAU - Petrosian, A
AU  - Petrosian A
FAU - Galera, O A
AU  - Galera OA
FAU - Wang, S J
AU  - Wang SJ
FAU - Jordan, S C
AU  - Jordan SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Cytokines)
RN  - 0 (DNA Probes)
RN  - 0 (Immunoglobulins)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Pokeweed Mitogens)
RN  - 0 (RNA, Messenger)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/*genetics/*immunology
MH  - DNA Probes
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Humans
MH  - Immunoglobulins/*biosynthesis
MH  - Immunoglobulins, Intravenous/*immunology
MH  - Lymphocyte Activation
MH  - Lymphocytes/*immunology
MH  - Pokeweed Mitogens
MH  - RNA/isolation & purification
MH  - RNA, Messenger/*biosynthesis
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1007/BF01533367 [doi]
PST - ppublish
SO  - J Clin Immunol. 1994 May;14(3):178-89. doi: 10.1007/BF01533367.