PMID- 7523503
OWN - NLM
STAT- MEDLINE
DCOM- 19941110
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 153
IP  - 8
DP  - 1994 Oct 15
TI  - Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant 
      protein-1.
PG  - 3708-16
AB  - Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a 
      member of the beta chemokine gene family and a homologue of the human monocyte 
      chemoattractant protein, MCP-1. Mouse rMCP-1 was used to immunize hamsters for 
      the production of mAb. Seven mouse MCP-1-specific mAbs were characterized: two of 
      these mAbs cross-reacted with the human MCP-1, as determined by ELISA. A 
      sensitive and specific capture ELISA for MCP-1 quantitation, which allowed 
      measurement of mouse MCP-1 levels in supernatants from cells stimulated with 
      inflammatory agents, was developed. LPS-stimulated astrocytes produce the highest 
      levels of MCP-1 (80 ng/ml); macrophages and mesangial cells produce lower levels 
      of MCP-1 (2 to 14 ng/ml) after LPS stimulation. IL-1 and TNF-alpha stimulation 
      also can induce low levels of MCP-1 production. Western blot analysis 
      demonstrated that the predominant native form of mouse MCP-1 is a 30-kDa 
      glycoprotein. Two mAbs (2H5 and 6C7) demonstrated dose-dependent neutralization 
      of mouse MCP-1 chemotactic activity. To localize the epitope recognized by one of 
      these neutralizing Abs, the mAb was used to bind a series of genetically 
      engineered truncated variants of human MCP-1. The C-terminal residues 62 to 67 on 
      human MCP-1 molecules seem to be critical to express the epitope recognized by 
      the neutralizing 2H5 anti-MCP-1 mAb. However, multiple sites on the MCP-1 
      molecule seem to be critical for bioactivity. Thus, these Ab reagents provide a 
      useful tool to explore the biology of the mouse MCP-1 beta chemokine.
FAU - Luo, Y
AU  - Luo Y
AD  - Department of Pathology, Harvard Medical School, Boston, MA 02115.
FAU - Laning, J
AU  - Laning J
FAU - Hayashi, M
AU  - Hayashi M
FAU - Hancock, P R
AU  - Hancock PR
FAU - Rollins, B
AU  - Rollins B
FAU - Dorf, M E
AU  - Dorf ME
LA  - eng
GR  - CA-56057/CA/NCI NIH HHS/United States
GR  - NS31152/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*immunology/metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Epitope Mapping
MH  - Epitopes
MH  - Female
MH  - Inflammation/immunology
MH  - Macrophage Activation
MH  - Mice
EDAT- 1994/10/15 00:00
MHDA- 1994/10/15 00:01
CRDT- 1994/10/15 00:00
PHST- 1994/10/15 00:00 [pubmed]
PHST- 1994/10/15 00:01 [medline]
PHST- 1994/10/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1994 Oct 15;153(8):3708-16.