PMID- 7523671
OWN - NLM
STAT- MEDLINE
DCOM- 19941025
LR  - 20201214
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 21
IP  - 6
DP  - 1994 Jun
TI  - Autoimmune sera react with multiple epitopes on recombinant 52 and 60 kDa Ro(SSA) 
      proteins.
PG  - 1073-80
AB  - OBJECTIVE: To determine the reactivity of recombinant 52 and 60 kDa Ro(SSA) (Ro) 
      proteins with sera from 3 subsets of patients with Ro autoantibody associated 
      disease. METHODS: Complementary DNA (cDNA) clones that encode the human 52 and 60 
      kDa Ro autoantigens were isolated by the polymerase chain reaction and utilized 
      to express recombinant glutathione S-transferase (GST) fusion proteins. Double 
      immunodiffusion (ID) defined Ro positive autoimmune sera from 12 patients with 
      neonatal lupus erythematosus (NLE), 16 with subacute cutaneous lupus 
      erythematosus (SCLE) and 12 with primary Sjogren's syndrome (SS) were tested by 
      enzyme linked immunosorbent assay (ELISA) against the recombinant 52 and 60 kDa 
      Ro fusion proteins. RESULTS: Seventy-five percent of NLE, 56% of SCLE and 83% of 
      SS sera reacted with the 52 kDa fusion protein. Seventy-five percent of NLE, 63% 
      of SCLE and 83% of SS sera reacted with the 60 kDa fusion protein. Seventeen 
      percent of NLE sera, 25% of SCLE sera and 8% of SS sera were nonreactive to both 
      full length fusion proteins. Eight (57%) of 14 ID defined Ro negative NLE, SCLE 
      and SS sera were reactive with both Ro fusion proteins by ELISA: ELISA studies 
      with recombinant 52 and 60 kDa Ro protein fragments revealed at least 2 major 
      epitopes on each Ro protein. A fragment of the 52 kDa Ro protein that contains a 
      putative leucine zipper motif reacted with 100% of ID defined Ro positive SS 
      sera. CONCLUSION: Our data demonstrate that the ID assay and the recombinant Ro 
      ELISA together are more sensitive in detecting Ro antibodies than either assay 
      alone, and that multiple epitopes are present on both Ro proteins.
FAU - McCauliffe, D P
AU  - McCauliffe DP
AD  - Department of Dermatology, University of North Carolina at Chapel Hill 27599.
FAU - Yin, H
AU  - Yin H
FAU - Wang, L X
AU  - Wang LX
FAU - Lucas, L
AU  - Lucas L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Autoantigens)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (Immune Sera)
RN  - 0 (RNA, Small Cytoplasmic)
RN  - 0 (RO60 protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antigen)
SB  - IM
MH  - Autoantigens/chemistry/*immunology
MH  - *Autoimmunity
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Epitopes
MH  - Humans
MH  - Immune Sera/*immunology
MH  - Infant, Newborn
MH  - Infant, Newborn, Diseases/immunology
MH  - Lupus Erythematosus, Cutaneous/immunology
MH  - Lupus Erythematosus, Systemic/immunology
MH  - Molecular Weight
MH  - Polymerase Chain Reaction
MH  - *RNA, Small Cytoplasmic
MH  - Recombinant Fusion Proteins/*immunology
MH  - Ribonucleoproteins/chemistry/*immunology
MH  - Sjogren's Syndrome/immunology
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1994 Jun;21(6):1073-80.