PMID- 7524063
OWN - NLM
STAT- MEDLINE
DCOM- 19941104
LR  - 20190904
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 9
IP  - 4
DP  - 1994 Jul
TI  - Expression of the ras-related rab3a gene in human insulinomas and normal human 
      pancreatic islets.
PG  - 434-8
AB  - To determine the expression of the small-molecular-weight guanosine 
      5'-triphosphate (GTP)-binding protein rab3a in human endocrine pancreatic tissue, 
      total RNA was isolated from five different human insulinomas and from normal 
      human islets. The expression of rab3a was analyzed by Northern blots utilizing a 
      1,300-bp Eco RI fragment of the cDNA coding for human rab3a. As in the brain, a 
      specific transcript of 1,800 bp was detected in all insulinomas, though with 
      varying signal intensity. When the northern membranes were rehybridized with a 
      radioactively labeled insulin probe, a signal was found for all five insulinomas 
      and cells of normal islets. In contrast, normal human islets showed no detectable 
      expression of rab3a. In conclusion, rab3a is expressed to a significant degree in 
      human insulinoma tissue but not in normal islets.
FAU - Lankat-Buttgereit, B
AU  - Lankat-Buttgereit B
AD  - Department of Internal Medicine, Philipps University of Marburg, Germany.
FAU - Fehmann, H C
AU  - Fehmann HC
FAU - Hering, B J
AU  - Hering BJ
FAU - Bretzel, R G
AU  - Bretzel RG
FAU - Goke, B
AU  - Goke B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (RNA, Neoplasm)
RN  - 63231-63-0 (RNA)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rab3 GTP-Binding Proteins)
SB  - IM
GS  - rab3a
MH  - Animals
MH  - Blotting, Northern
MH  - GTP-Binding Proteins/biosynthesis/*genetics
MH  - Gene Expression
MH  - Genes, ras/*genetics
MH  - Humans
MH  - Insulinoma/*genetics
MH  - Islets of Langerhans/*metabolism
MH  - Pancreatic Neoplasms/*genetics
MH  - RNA/analysis/genetics/isolation & purification
MH  - RNA, Neoplasm/analysis/genetics/isolation & purification
MH  - Rats
MH  - rab3 GTP-Binding Proteins
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1097/00006676-199407000-00004 [doi]
PST - ppublish
SO  - Pancreas. 1994 Jul;9(4):434-8. doi: 10.1097/00006676-199407000-00004.