PMID- 7526009
OWN - NLM
STAT- MEDLINE
DCOM- 19941227
LR  - 20190821
IS  - 0741-5214 (Print)
IS  - 0741-5214 (Linking)
VI  - 20
IP  - 5
DP  - 1994 Nov
TI  - Cellular localization of matrix metalloproteinases in the abdominal aortic 
      aneurysm wall.
PG  - 814-20
AB  - PURPOSE: This study explores the source(s) of the matrix-degrading proteinases, 
      matrix metalloproteinase 1 (MMP-1; interstitial collagenase), matrix 
      metalloproteinase 3 (MMP-3; stromelysin 1), and matrix metalloproteinase 9 
      (MMP-9; gelatinase B), previously implicated in abdominal aortic aneurysm (AAA) 
      development. The possible involvement of the plasmin cascade in the activation of 
      these proteinases was also explored by examining the presence of the 
      urokinase-type plasminogen activator (uPA) in aneurysm wall. METHODS: 
      Immunohistochemical techniques were used to detect the presence of MMP-1, MMP-3 
      and MMP-9 proteins and uPA in fixed, paraffin-embedded tissue sections from AAA 
      (n = 10) and control (n = 2) aortas. RESULTS: The MMP-9 protein was localized to 
      mononuclear cells in the AAA wall. Dual-labeling techniques confirmed the 
      identity of these cells as macrophages. The MMP-3 protein and uPA were also 
      detected primarily in the macrophage-like mononuclear cells infiltrating the 
      aneurysmal aorta. Immunoreactive material to MMP-1 was demonstrated in 
      mesenchymal cells of the AAA wall suggesting alternative expression and delivery 
      of this enzyme in AAA. CONCLUSIONS: This work establishes the role of macrophages 
      in the delivery, expression, and possible activation of matrix destructive 
      proteinases during AAA pathogenesis and suggests a role for the activation of 
      MMPs in the progression of the disease.
FAU - Newman, K M
AU  - Newman KM
AD  - Department of Surgery, St. Lukes/Roosevelt Hospital Center, New York, NY 10019.
FAU - Jean-Claude, J
AU  - Jean-Claude J
FAU - Li, H
AU  - Li H
FAU - Scholes, J V
AU  - Scholes JV
FAU - Ogata, Y
AU  - Ogata Y
FAU - Nagase, H
AU  - Nagase H
FAU - Tilson, M D
AU  - Tilson MD
LA  - eng
GR  - AR39189/AR/NIAMS NIH HHS/United States
GR  - HL29325/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Vasc Surg
JT  - Journal of vascular surgery
JID - 8407742
RN  - 0 (Antibodies, Monoclonal)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Aortic Aneurysm, Abdominal/*enzymology/pathology
MH  - Collagenases/*metabolism
MH  - Culture Techniques
MH  - Endothelium, Vascular/*metabolism/pathology/ultrastructure
MH  - Humans
MH  - Macrophages/*metabolism/pathology
MH  - Matrix Metalloproteinase 1
MH  - Matrix Metalloproteinase 3
MH  - Matrix Metalloproteinase 9
MH  - Metalloendopeptidases/*metabolism
MH  - Staining and Labeling
MH  - Urokinase-Type Plasminogen Activator/*metabolism
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - S0741-5214(94)70169-5 [pii]
AID - 10.1016/s0741-5214(94)70169-5 [doi]
PST - ppublish
SO  - J Vasc Surg. 1994 Nov;20(5):814-20. doi: 10.1016/s0741-5214(94)70169-5.