PMID- 7526337
OWN - NLM
STAT- MEDLINE
DCOM- 19941129
LR  - 20190726
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 428
IP  - 1
DP  - 1994 Aug
TI  - Volume regulatory responses in frog isolated proximal cells.
PG  - 60-8
AB  - Cells respond to increase in volume by activating solute efflux pathways, 
      resulting in water loss and restoration of the original cell volume. The solute 
      efflux pathways underlying these volume regulatory decrease (VRD) responses have 
      been relatively well studied. However, the transduction pathways whereby the 
      change in cell volume is converted into an intracellular signal resulting in VRD 
      are much less well understood. We have examined VRD in isolated proximal tubule 
      cells from the frog, with particular attention to the roles of stretch-activated 
      channels, Ca2+ and protein kinases. Cell length was taken as an index of cell 
      volume, and was measured continuously using a photodiode array. VRD was observed 
      in approximately 50% of cells, and was inhibited by Ba2+, Gd3+ and 
      4,4'-diisothiocyanatostilbene 2,2'-disulphonic acid (DIDS), and removal of 
      extracellular Ca2+. VRD was accelerated by the active phorbol ester, phorbol 
      12-myristate 13-acetate (PMA), and the phosphatase inhibitor F-; on the other 
      hand, VRD was prolonged by 4 alpha-phorbol 12,13-didecanoate (PDC), an inactive 
      phorbol ester), and inhibited by PMA and Gd3+, PMA and 0 Ca2+, and staurosporine. 
      Volume regulation was unaffected by di-butyryl cAMP and 
      3-isobutyl-1-methyl-xanthene (IBMX). These data suggest that Ca2+ and PKC, via 
      protein phosphorylation, play a stimulatory role in VRD.
FAU - Robson, L
AU  - Robson L
AD  - Department of Physiology, Medical School, Leeds University, UK.
FAU - Hunter, M
AU  - Hunter M
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Calcium Channels)
RN  - 0 (Chloride Channels)
RN  - 0 (Ion Channels)
RN  - 0 (Potassium Channels)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 24GP945V5T (Barium)
SB  - IM
MH  - Animals
MH  - Barium/metabolism
MH  - Calcium Channels/metabolism
MH  - Cell Size/physiology
MH  - Chloride Channels/metabolism
MH  - In Vitro Techniques
MH  - Ion Channels/*metabolism
MH  - Kidney Tubules, Proximal/cytology/*metabolism
MH  - Osmolar Concentration
MH  - Phosphorylation
MH  - Potassium Channels/metabolism
MH  - Protein Kinase Inhibitors
MH  - Rana temporaria
MH  - Signal Transduction/physiology
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1007/BF00374752 [doi]
PST - ppublish
SO  - Pflugers Arch. 1994 Aug;428(1):60-8. doi: 10.1007/BF00374752.