PMID- 7527913
OWN - NLM
STAT- MEDLINE
DCOM- 19950119
LR  - 20190913
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 25
IP  - 6
DP  - 1994 Dec
TI  - Loss and gain of chromosomes 1, 18, and Y in prostate cancer.
PG  - 281-91
AB  - Nuclear suspensions of 42 prostate carcinoma specimens obtained at surgery were 
      used to investigate loss and gain chromosomes 1, 18, and Y by fluorescence in 
      situ hybridization (FISH) with centromere-specific probes. The outcome of FISH 
      analysis was correlated with clinical parameters and the relationship between 
      DNA-FCM (ploidy at cellular level) and FISH (ploidy of individual chromosomes) 
      was assessed. Significant loss of chromosomes 1 and 18 was infrequent 
      (respectively, three and five cases), but 53% of the tested specimens showed loss 
      of Y. Loss was not correlated with DNA ploidy. Significant gain occurred in 36% 
      (chromosome 1), 63% (chromosome 18), and 28% (Y) of the specimens. Gain of 
      chromosome 18 was shown in DNA diploid (7/14) and aneuploid tumors (18/26), while 
      gain of chromosomes 1 and Y was nearly restricted to DNA aneuploid specimens. 
      Significant unbalance between these chromosomes occurred in 11 cases. Most cases 
      which had significant gain of chromosome 1 or 18 showed trisomic as well as 
      tetrasomic cells. Simultaneous loss of some and gain of other investigated 
      chromosomes is suggestive of clonal heterogeneity and/or multiclonality. This was 
      observed in eight tumors. Correlation between DNA-FCM and FISH was best for the Y 
      chromosome. DNA-FCM showed more aberrant histograms with increasing stage and 
      grade of tumors. The presence of numerical aberrations of the investigated 
      chromosomes however, seemed independent of clinical grade or stage.
FAU - Konig, J J
AU  - Konig JJ
AD  - Department of Urology, Erasmus University, Rotterdam, The Netherlands.
FAU - Teubel, W
AU  - Teubel W
FAU - van Dongen, J W
AU  - van Dongen JW
FAU - Romijn, J C
AU  - Romijn JC
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Schroder, F H
AU  - Schroder FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 18
MH  - DNA, Neoplasm
MH  - Humans
MH  - Male
MH  - Ploidies
MH  - Prostatic Hyperplasia/genetics
MH  - Prostatic Neoplasms/*genetics
MH  - *Y Chromosome
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1002/pros.2990250602 [doi]
PST - ppublish
SO  - Prostate. 1994 Dec;25(6):281-91. doi: 10.1002/pros.2990250602.