PMID- 7528245
OWN - NLM
STAT- MEDLINE
DCOM- 19950124
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 104
IP  - 1
DP  - 1995 Jan
TI  - Differential responsiveness of Langerhans cell subsets of varying phenotypic 
      states in normal human epidermis.
PG  - 42-6
AB  - Epidermal Langerhans cell heterogeneity is poorly understood with regard to 
      phenotypic characteristics, such as the expression of human leukocyte antigen 
      (HLA)-DR, integrin, and Fc receptor molecules, as well as functional 
      characteristics, such as the ability to process and present antigens or produce 
      cytokines during various phases of immigration and maturation. Technical 
      limitations of Langerhans cell number have limited functional assays on putative 
      Langerhans cell subsets in in vivo epidermis. Therefore, we used flow cytometry 
      for simultaneous phenotypic and functional assessment at the single-cell level 
      within the Langerhans cell population. Freshly isolated human epidermal cell 
      suspensions were stained with a battery of monoclonal antibodies, including 
      anti-HLA-DR, -CD1a, -CD1c, -CD11c, -Fc gamma RII, and -Fc epsilon RI. Two 
      distinct Langerhans cell subsets were identified by their different levels of 
      HLA-DR expression. The DRHi subset expressed higher amounts of CD11c and 
      exhibited greater cytoplasmic complexity and higher baseline calcium than the 
      DRLo subset (p < or = 0.03 for each). Some subjects also expressed high levels of 
      Fc epsilon RI in the DRHi, CD11cHi subset. To determine whether these phenotypic 
      subsets may exhibit differential signal-transduction functional properties, 
      Langerhans cells were partially enriched over Ficoll-Hypaque and their cytosolic 
      mobilization after the addition of ionomycin was analyzed using the calcium 
      indicator, indo-1, in conjunction with quantitative analysis of HLA-DR 
      expression. By this real-time flow cytometric analysis, a new subpopulation was 
      revealed within the DRLo Langerhans cell subset. This subset increased its 
      cytosolic calcium concentration much more than the other two subsets (change in 
      indo-1 blue:violet emission ratio of 37.33 +/- 2.34 in the Hi Flux DRLo subset 
      versus 13.23 +/- 0.29 in the Lo Flux DRLo subset, and versus 7.6 +/- 2.99 in the 
      Lo Flux DRHi subset). These data indicate that functional, as well as phenotypic, 
      subsets of Langerhans cells exist within normal human epidermis. Their responses 
      to physiologic stimuli may relate to maturational stage or the level of in vivo 
      activation.
FAU - Shibaki, A
AU  - Shibaki A
AD  - Department of Dermatology, University of Michigan Medical School, Ann Arbor.
FAU - Meunier, L
AU  - Meunier L
FAU - Ra, C
AU  - Ra C
FAU - Shimada, S
AU  - Shimada S
FAU - Ohkawara, A
AU  - Ohkawara A
FAU - Cooper, K D
AU  - Cooper KD
LA  - eng
GR  - P60 AR20557/AR/NIAMS NIH HHS/United States
GR  - RD-1 AR 41642-01A1/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (CD18 Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Integrins)
RN  - 0 (Receptors, Fc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Antigens, CD1
MH  - CD18 Antigens
MH  - Calcium/metabolism
MH  - Cytosol/metabolism
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Integrins/analysis
MH  - Langerhans Cells/chemistry/*classification/*physiology
MH  - Phenotype
MH  - Receptors, Fc/analysis
MH  - Skin/ultrastructure
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - S0022-202X(15)42000-7 [pii]
AID - 10.1111/1523-1747.ep12613476 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1995 Jan;104(1):42-6. doi: 10.1111/1523-1747.ep12613476.