PMID- 7528950
OWN - NLM
STAT- MEDLINE
DCOM- 19950202
LR  - 20071114
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 58
IP  - 12
DP  - 1994 Dec 27
TI  - Divergent patterns of ELAM-1, ICAM-1, and VCAM-1 expression on 
      cytomegalovirus-infected endothelial cells.
PG  - 1379-85
AB  - Endothelial cells, one of several in vivo host cells for cytomegalovirus (CMV), 
      participate in solid organ allograft rejection in part through the expression of 
      leukocyte adhesion molecules. The hypothesis that CMV infection alters the 
      constitutive and induced expression of ELAM-1, ICAM-1, and VCAM-1 on infected 
      human umbilical vein endothelial cells (HUVECs) was examined. HUVECs were 
      infected with an endothelial cell-propagated strain of CMV (VHL/E) for various 
      periods, treated with tumor necrosis factor-alpha (TNF alpha), and examined by 
      flow cytometry or immunohistochemically dual-labeled with monoclonal antibodies 
      to CMV immediate early nuclear protein and ELAM-1, ICAM-1, or VCAM-1. Neither 
      ELAM-1 nor VCAM-1 was induced on CMV-infected HUVECs, and treatment with TNF 
      alpha treatment did not result in their induction. In contrast, ICAM-1 was 
      induced on infected HUVECs by 24 hr postinfection. Endothelial ICAM-1 induction 
      may represent a mechanism by which CMV infection exacerbates the recipient 
      cellular immune response to allografts.
FAU - Sedmak, D D
AU  - Sedmak DD
AD  - Department of Pathology, Ohio State University College of Medicine, Columbus.
FAU - Knight, D A
AU  - Knight DA
FAU - Vook, N C
AU  - Vook NC
FAU - Waldman, J W
AU  - Waldman JW
LA  - eng
GR  - AI29002/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (E-Selectin)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Antibodies, Viral/analysis
MH  - Cell Adhesion Molecules/*analysis/immunology/physiology
MH  - Cells, Cultured
MH  - Cytomegalovirus/immunology/*isolation & purification/metabolism
MH  - Cytomegalovirus Infections/metabolism
MH  - E-Selectin
MH  - Endothelium, Vascular/*chemistry/*cytology/virology
MH  - Flow Cytometry
MH  - Humans
MH  - Immediate-Early Proteins/immunology/metabolism
MH  - Immunohistochemistry
MH  - Intercellular Adhesion Molecule-1/*analysis/immunology/physiology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Cell Adhesion Molecule-1
EDAT- 1994/12/27 00:00
MHDA- 1994/12/27 00:01
CRDT- 1994/12/27 00:00
PHST- 1994/12/27 00:00 [pubmed]
PHST- 1994/12/27 00:01 [medline]
PHST- 1994/12/27 00:00 [entrez]
PST - ppublish
SO  - Transplantation. 1994 Dec 27;58(12):1379-85.