PMID- 7530482
OWN - NLM
STAT- MEDLINE
DCOM- 19950227
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 11
IP  - 3
DP  - 1994 Nov
TI  - Y chromosome loss in chronic myeloid leukemia detected in both normal and 
      malignant cells by interphase fluorescence in situ hybridization.
PG  - 141-5
AB  - Loss of the Y chromosome in bone marrow (BM) cells is a normal age-associated 
      event. Y chromosome loss is also observed in the Philadelphia chromosome (Ph) 
      positive BM cells of some patients with chronic myeloid leukemia (CML) in chronic 
      phase, but at a younger age than in normal individuals. While the significance of 
      loss of the sex chromosome in normal males is uncertain, -Y marrow cells are not 
      believed to be of clonal origin. However, because CML is a clonal disease, CML 
      sub-populations with Y loss may constitute a disease-related sub-clone. We used a 
      PCR-amplified yeast artificial chromosome containing the BCR gene region for 
      single color interphase analysis of BCR rearrangement by fluorescence in situ 
      hybridization (FISH). Then, using two color FISH, with one fluorochrome detecting 
      the BCR gene region and the other detecting Y chromosome repeat sequences, we 
      surveyed peripheral and BM Y loss in both normal Ph- (BCR not disrupted) and CML 
      Ph+ (BCR rearranged) interphase nuclei of two patients with Y loss in Ph positive 
      cells observed by metaphase analysis. -Y was seen in a proportion of Ph+ cells in 
      both cases, and the proportion matched that seen in Ph- cells, indicating that Y 
      loss is probably sporadic in both normal and CML populations, and that the 
      propensity for Y loss in normal BM cells may be a phenotype that can be retained 
      by malignant cells in CML.
FAU - Kirk, J A
AU  - Kirk JA
AD  - Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
FAU - VanDevanter, D R
AU  - VanDevanter DR
FAU - Biberman, J
AU  - Biberman J
FAU - Bryant, E M
AU  - Bryant EM
LA  - eng
GR  - CA 18029/CA/NCI NIH HHS/United States
GR  - CA 54998/CA/NCI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
GS  - BCR
MH  - *Chromosome Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Y Chromosome
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1002/gcc.2870110302 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1994 Nov;11(3):141-5. doi: 10.1002/gcc.2870110302.