PMID- 7530605
OWN - NLM
STAT- MEDLINE
DCOM- 19950227
LR  - 20190512
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 16
IP  - 1
DP  - 1995 Jan
TI  - TGF alpha differentially expressed in liver foci induced by diethylnitrosamine 
      initiation and peroxisome proliferator promotion.
PG  - 77-82
AB  - Transforming growth factor alpha (TGF alpha) is a mitogenic growth factor for 
      hepatocytes that may play a role in the development of liver cancer in rodents 
      and humans. Epidermal growth factor receptor (EGFR) is the receptor for TGF 
      alpha; its expression is also altered in mitogenic and carcinogenic processes. 
      Homogeneous basophilic foci (HBF), the precursor lesion to hepatocellular 
      carcinomas in peroxisome proliferator (PP)-treated rats, have labeling indices 
      much greater than surrounding liver (approximately 5- to 20-fold) and other types 
      of foci (approximately 2-fold greater than eosinophilic foci; EF). To test the 
      hypothesis that PP-induced HBF over-express TGF alpha and/or EGFR, male F344 rats 
      were treated with the PP WY-14,643 for 22 weeks (1000 p.p.m. in the diet) with 
      (DEN-WY) and without (WY) prior diethylnitrosamine initiation (150 mg/kg body wt 
      i.p.). Serial paraffin sections of liver were stained for TGF alpha or EGFR and 
      with hematoxylin and eosin. DEN-WY and WY abrogated the small amount of 
      centrilobular TGF alpha staining observed in livers from control rats. Increased 
      staining for TGF alpha was not observed in HBF induced by WY (0/22) or DEN-WY 
      (0/101). Additionally, increased EGFR expression was not observed in HBF induced 
      by WY (0/22) or DEN-WY (0/19) or in EF induced by DEN-WY (0/30). An unexpectedly 
      large proportion of EF induced by DEN-WY (6/38) and DEN-control (3/11) were TGF 
      alpha-positive. None of the tumors induced by WY (0/13) or DEN-WY (0/11) 
      over-expressed TGF alpha. All 13 WY-induced tumors also lacked increased 
      expression of EGFR. TGF alpha over-expression noted in a significant proportion 
      of EF was associated only with those regimens including DEN initiation. In 
      conclusion, TGF alpha or EGFR over-expression is not associated with early 
      appearing, rapidly proliferating HBF or tumors induced by PP.
FAU - Miller, R T
AU  - Miller RT
AD  - Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709.
FAU - Cattley, R C
AU  - Cattley RC
FAU - Marsman, D S
AU  - Marsman DS
FAU - Lyght, O
AU  - Lyght O
FAU - Popp, J A
AU  - Popp JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Carcinogens)
RN  - 0 (Growth Substances)
RN  - 0 (Pyrimidines)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
RN  - 86C4MRT55A (pirinixic acid)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Carcinogens
MH  - Cell Division/drug effects/physiology
MH  - Diethylnitrosamine
MH  - ErbB Receptors/physiology
MH  - Growth Substances/physiology
MH  - Liver/chemistry/drug effects
MH  - Liver Neoplasms, Experimental/chemically induced/*pathology/ultrastructure
MH  - Male
MH  - Microbodies/*drug effects
MH  - Pyrimidines
MH  - Rats
MH  - Rats, Inbred F344
MH  - Signal Transduction/drug effects/physiology
MH  - Staining and Labeling/methods
MH  - Transforming Growth Factor alpha/analysis/*physiology
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1093/carcin/16.1.77 [doi]
PST - ppublish
SO  - Carcinogenesis. 1995 Jan;16(1):77-82. doi: 10.1093/carcin/16.1.77.