PMID- 7531718
OWN - NLM
STAT- MEDLINE
DCOM- 19950310
LR  - 20061115
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 80
IP  - 2
DP  - 1995 Feb
TI  - Human histocompatibility leukocyte antigen-DR and heat shock protein-70 
      expression in eye muscle tissue in thyroid-associated ophthalmopathy.
PG  - 685-91
AB  - To investigate the expression and localization of human leukocyte antigen 
      (HLA)-DR and heat shock protein-70 (HSP-70) in orbital tissue from patients with 
      thyroid-associated ophthalmopathy (TAO), we carried out an immunohistochemical 
      study using anti-HLA-DR and anti-HSP-70 monoclonal antibodies and a 
      streptavidin-biotinperoxidase detection system. Eye muscle tissues were obtained 
      at surgery from 38 patients with TAO and 8 control subjects. HLA-DR expression on 
      eye muscle cells was demonstrated in orbital tissue from 2 of 3 untreated 
      patients and 2 of 35 patients who had been treated with orbital irradiation or 
      corticosteroids, in all of whom lymphocytic infiltration was also demonstrated. 
      HLA-DR was not detected on eye muscle cells from 8 normal controls studied. 
      HLA-DR was expressed on endothelial cells and interstitial cells from almost all 
      patients with TAO and all 8 control subjects. HSP-70 was detected in eye muscle 
      cells from 31 of the patients with TAO, including all 3 untreated patients, and 3 
      of the controls. Although the degree of HSP-70 expression did not correlate with 
      the severity of the ophthalmopathy, significant expression of HSP-70 in eye 
      muscle cells was more often demonstrated in patients with eye disease of short 
      duration (83%) than in those with disease of longer duration (33%). These results 
      support the notion that eye muscle fiber is an important target of the orbital 
      autoimmune reactions that characterize TAO.
FAU - Hiromatsu, Y
AU  - Hiromatsu Y
AD  - Department of Medicine, Kurume University School of Medicine, Japan.
FAU - Tanaka, K
AU  - Tanaka K
FAU - Ishisaka, N
AU  - Ishisaka N
FAU - Kamachi, J
AU  - Kamachi J
FAU - Kuroki, T
AU  - Kuroki T
FAU - Hoshino, T
AU  - Hoshino T
FAU - Inoue, Y
AU  - Inoue Y
FAU - Wall, J R
AU  - Wall JR
FAU - Nonaka, K
AU  - Nonaka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HSP70 Heat-Shock Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Eye Diseases/*etiology/*metabolism/therapy
MH  - Female
MH  - HLA-DR Antigens/*metabolism
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Oculomotor Muscles/*metabolism/pathology
MH  - Staining and Labeling
MH  - Thyroid Diseases/*complications
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1210/jcem.80.2.7531718 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1995 Feb;80(2):685-91. doi: 10.1210/jcem.80.2.7531718.