PMID- 7532367
OWN - NLM
STAT- MEDLINE
DCOM- 19950321
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 268
IP  - 2 Pt 1
DP  - 1995 Feb
TI  - TGF-beta regulates expression of tenascin alternative-splicing isoforms in fetal 
      rat lung.
PG  - L173-80
AB  - Two distinct mRNA-splice isoforms of tenascin (TN) are expressed differentially 
      during rat lung development. The unique temporal expression pattern of two TN 
      isoforms suggests the expression of tenascin is strictly developmentally 
      regulated in rat lung tissue. We investigated molecular mechanisms which modulate 
      alternative-splicing expression of TN in lung development. The effect of 
      transforming growth factor-beta 1 (TGF-beta 1) on regulation of expression of TN 
      isoforms was examined by in vitro lung explant culture. Immunoblotting with 
      anti-TN antibody detected two TN polypeptides in rat lung explant culture, the 
      larger [relative molecular weight (M(r)) 230, TN230] polypeptide and the smaller 
      (M(r) 180, TN180). TGF-beta 1 markedly induced the TN180 isoform and caused only 
      a moderate increase of the TN230 isoform. The effects of TGF-beta 1 were shown to 
      be dose dependent over a physiological range of TGF-beta 1 protein concentration. 
      The induction of TN isoform biosynthesis by TGF-beta 1 was detected 12 h after 
      addition of the growth factor, and the effects endured for up to 48 h at a dose 
      of 5 ng/ml. By reverse transcriptase-polymerase chain reaction through 
      amplification of the entire fibronectin type III (FN-III) splicing domain, two 
      distinct TN isoforms were detected in total RNA isolated from gestational day 21 
      rat lung explant culture treated with TGF-beta 1 and from postnatal day 8 rat 
      lung. The larger isoform contained five FN-III alternative splicing repeats 
      [1,420 base pairs (bp)], but the shorter splicing isoform lacked four FN-III 
      alternative splicing repeats (340 bp).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Zhao, Y
AU  - Zhao Y
AD  - Research Service, Durham Department of Veterans Affairs Medical Center, North 
      Carolina 27705.
FAU - Young, S L
AU  - Young SL
LA  - eng
GR  - HL-32188/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Tenascin)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Cell Adhesion Molecules, Neuronal/chemistry/*genetics/*metabolism
MH  - Embryonic and Fetal Development
MH  - Extracellular Matrix Proteins/chemistry/*genetics/*metabolism
MH  - Fetus/*metabolism/physiology
MH  - Isomerism
MH  - Lung/*embryology
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tenascin
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1152/ajplung.1995.268.2.L173 [doi]
PST - ppublish
SO  - Am J Physiol. 1995 Feb;268(2 Pt 1):L173-80. doi: 10.1152/ajplung.1995.268.2.L173.