PMID- 7532755
OWN - NLM
STAT- MEDLINE
DCOM- 19950330
LR  - 20190724
IS  - 0160-2446 (Print)
IS  - 0160-2446 (Linking)
VI  - 24
IP  - 5
DP  - 1994 Nov
TI  - Effect of hemicholinium-3 on the hypothalamic concentration of a cytochemically 
      detectable glucose-6-phosphate dehydrogenase-stimulating substance.
PG  - 773-8
AB  - Hypothalamus and plasma of salt-loaded rats, spontaneously hypertensive rats 
      (SHR), and hypertensive reduced renal mass rats (RRM), and the plasma of patients 
      with essential hypertension and of Milan hypertensive rats contain an increased 
      concentration of a cytochemically detectable glucose-6-phosphate dehydrogenase 
      (G6PD)-stimulating substance that has properties similar to that of a possible 
      choline derivative di-methyl methylene immonium ion. Intracerebroventricular 
      (i.c.v.) administration of hemicholinium-3 (HC-3) selectively blocks 
      high-affinity neuronal choline uptake, inhibits brain acetylcholine (ACh) 
      synthesis, and decreases arterial pressure in SHR through an inhibiting effect on 
      hypothalamic cholinergic function. The experiments were performed to study the 
      effect of centrally administered HC-3 on the content of the cytochemically 
      detectable cholinelike substance in hypothalamus and plasma of SHR. HC-3 or 
      saline was infused into the lateral cerebral ventricle for 6 days with a minipump 
      in 14 SHR. On day 7, the hypothalamic and plasma concentration of the 
      cytochemically detectable substance was significantly reduced in rats that 
      received HC-3. The hypothalamic concentration was 225 +/- 95.6 x 10(8) G6PD U per 
      hypothalamus (range 38.2-775) in SHR that received saline and 1.037 +/- 0.45 x 
      10(8) G6PD U (range 0.112-3.61) (p < 0.05) in SHR that received HC-3. The 
      respective plasma concentrations were 284.9 +/- 26 U/ml (range 192-374) and 72.7 
      +/- 14.7 U/ml (range 24-119) (p < 0.05). The findings are consistent with the 
      physicochemical evidence, which suggests that the cytochemically detectable 
      substance is a choline derivative.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Vargas, H M
AU  - Vargas HM
AD  - Biological Research Department, Hoechst-Roussel Pharmaceutical, Somerville, New 
      Jersey.
FAU - Brezenoff, H E
AU  - Brezenoff HE
FAU - Morris, H R
AU  - Morris HR
FAU - Panico, M
AU  - Panico M
FAU - Etienne, A
AU  - Etienne A
FAU - Challand, G S
AU  - Challand GS
FAU - Holland, S M
AU  - Holland SM
FAU - Alaghband Zadeh, J
AU  - Alaghband Zadeh J
FAU - de Wardener, H E
AU  - de Wardener HE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Cardiovasc Pharmacol
JT  - Journal of cardiovascular pharmacology
JID - 7902492
RN  - 312-45-8 (Hemicholinium 3)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - N91BDP6H0X (Choline)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Animals
MH  - Blood Pressure/*drug effects
MH  - Choline/metabolism
MH  - Enzyme Activation
MH  - Glucosephosphate Dehydrogenase/blood/*metabolism
MH  - Guinea Pigs
MH  - Hemicholinium 3/administration & dosage/*pharmacology/therapeutic use
MH  - Histocytochemistry
MH  - Humans
MH  - Hypertension/*drug therapy/enzymology
MH  - Hypothalamus/*drug effects/metabolism
MH  - Infusion Pumps, Implantable
MH  - Injections, Intraventricular
MH  - Kidney/enzymology
MH  - Male
MH  - Osmosis
MH  - Rats
MH  - Rats, Inbred SHR
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1097/00005344-199424050-00012 [doi]
PST - ppublish
SO  - J Cardiovasc Pharmacol. 1994 Nov;24(5):773-8. doi: 
      10.1097/00005344-199424050-00012.