PMID- 7533347
OWN - NLM
STAT- MEDLINE
DCOM- 19950406
LR  - 20171116
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 59
IP  - 4
DP  - 1995 Feb 27
TI  - Upregulation of cytokines associated with macrophage activation in the 
      Lewis-to-F344 rat transplantation model of chronic cardiac rejection.
PG  - 572-8
AB  - Lewis-to-F344 rat cardiac allografts develop chronic rejection and 
      arteriosclerotic lesions rich in mononuclear cells (especially macrophages). This 
      study was performed to determine whether cytokine pathways associated with 
      macrophage activation are upregulated in hearts undergoing chronic rejection. 
      Gene transcript levels for IFN-gamma, monocyte chemoattractant protein-1 (MCP-1), 
      and IL-6 were measured with reverse-transcription PCR assays optimized for each 
      gene. Gene products were confirmed by immunohistology. For all three genes, 
      transcript levels in rat cardiac allografts increased significantly on day 7 and 
      remained elevated on days 14 and 28 posttransplantation, as compared with naive 
      hearts, paired host hearts, and syngrafts (P < 0.006). For the inducible genes 
      IFN-gamma and MCP-1, high transcript levels in cardiac allografts were in 
      contrast with low levels in host spleens. On the other hand, transcript levels 
      for the basally expressed gene IL-6 were elevated in both organs. Immunostaining 
      confirmed allograft-specific expression for all three cytokines and localized the 
      gene products to infiltrating mononuclear cells in the interstitium and 
      vasculature. The sustained expression of these cytokines in cardiac allografts 
      undergoing chronic rejection supports the widely held hypothesis that the intimal 
      changes associated with transplant arteriosclerosis are mediated by cellular 
      activation and cytokine production.
FAU - Russell, M E
AU  - Russell ME
AD  - Harvard School of Public Health, Boston, Massachusetts.
FAU - Wallace, A F
AU  - Wallace AF
FAU - Hancock, W W
AU  - Hancock WW
FAU - Sayegh, M H
AU  - Sayegh MH
FAU - Adams, D H
AU  - Adams DH
FAU - Sibinga, N E
AU  - Sibinga NE
FAU - Wyner, L R
AU  - Wyner LR
FAU - Karnovsky, M J
AU  - Karnovsky MJ
LA  - eng
GR  - HL43318/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (Chemotactic Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-6)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 63231-63-0 (RNA)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CD4 Antigens/analysis
MH  - CD8 Antigens/analysis
MH  - Chemotactic Factors/*analysis
MH  - DNA, Complementary
MH  - Graft Rejection/*immunology
MH  - *Heart Transplantation
MH  - Interferon-gamma/*analysis
MH  - Interleukin-6/*analysis
MH  - Macrophage Activation
MH  - Molecular Sequence Data
MH  - Monocyte Chemoattractant Proteins
MH  - Myocardium/immunology
MH  - RNA/analysis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Inbred Lew
MH  - Transplantation, Homologous
EDAT- 1995/02/27 00:00
MHDA- 1995/02/27 00:01
CRDT- 1995/02/27 00:00
PHST- 1995/02/27 00:00 [pubmed]
PHST- 1995/02/27 00:01 [medline]
PHST- 1995/02/27 00:00 [entrez]
PST - ppublish
SO  - Transplantation. 1995 Feb 27;59(4):572-8.