PMID- 7534861
OWN - NLM
STAT- MEDLINE
DCOM- 19950425
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 27
IP  - 2
DP  - 1994 Dec
TI  - Stabilization of neurofilament transcripts during postnatal development.
PG  - 215-20
AB  - Neurofilament (NF) mRNAs in primary sensory neurons are long-lived transcripts 
      that undergo transcription-dependent destabilization when placed in primary 
      culture [32]. Destabilization of NF transcripts implies that the transcripts are 
      stabilized in high-expressing neurons and that stabilization may coordinate and 
      increase levels of NF expression. The present study examines the stabilities of 
      the three NF subunit mRNAs in postnatal cultures of dorsal root ganglia (DRG) to 
      determine whether increased stability of NF mRNAs could be responsible for the 
      coordinate postnatal upregulation of the three NF subunits [29]. The studies show 
      that the light (NF-L), mid-sized (NF-M) and heavy (NF-H) NF mRNAs are lost at 8 
      and 16 h in primary cultures from postnatal day 2 (P2) rats, but much less so in 
      cultures from postnatal day 16 (P16) and day 30 (P30) rats. Losses of each NF 
      mRNAs in P2 cultures occurs simultaneously in the presence or absence of 
      actinomycin. The findings support the view that stabilization of NF transcripts 
      contribute to the high and coordinate level NF expression and that components of 
      the stabilizing process are acquired during postnatal development.
FAU - Schwartz, M L
AU  - Schwartz ML
AD  - Division of Neuropathology, University of Pennsylvania Medical School, 
      Philadelphia 19104-6079.
FAU - Shneidman, P S
AU  - Shneidman PS
FAU - Bruce, J
AU  - Bruce J
FAU - Schlaepfer, W W
AU  - Schlaepfer WW
LA  - eng
GR  - K08 NS01451/NS/NINDS NIH HHS/United States
GR  - R01 NS15722/NS/NINDS NIH HHS/United States
GR  - T32 NS07064/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Actins)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (RNA, Messenger)
RN  - 1CC1JFE158 (Dactinomycin)
SB  - IM
MH  - Actins
MH  - Animals
MH  - Cells, Cultured
MH  - Dactinomycin/pharmacology
MH  - Ganglia, Spinal/physiology
MH  - Gene Expression/genetics
MH  - Neurofilament Proteins/*genetics
MH  - Neurons/physiology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Up-Regulation
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 0169-328X(94)90003-5 [pii]
AID - 10.1016/0169-328x(94)90003-5 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1994 Dec;27(2):215-20. doi: 
      10.1016/0169-328x(94)90003-5.