PMID- 7535187
OWN - NLM
STAT- MEDLINE
DCOM- 19950428
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 80
IP  - 1
DP  - 1995 Mar
TI  - Analysis of chromosomal abnormalities in human sperm after chemotherapy by 
      karyotyping and fluorescence in situ hybridization (FISH).
PG  - 29-32
AB  - The frequency of numerical and structural chromosomal abnormalities was studied 
      in the sperm of a lymphoma patient 3 years after MACOP-B chemotherapy (CT). Sperm 
      karyotyping was performed by fusion of human sperm with hamster oocytes and 
      analysis of 193 Q-banded sperm chromosomes. Multicolor fluorescence in situ 
      hybridization (FISH) was performed on 10,228 sperm for analysis of disomy 
      frequencies for chromosomes 1 and 12 and on 10,664 sperm for chromosomes X and Y. 
      Sperm karyotyping demonstrated numerical abnormalities in 7.3% of the spreads, 
      6.7% hypohaploid and 0.5% hyperhaploid, giving a conservative estimate of 
      aneuploidy of 1%. Structural chromosomal abnormalities were present in 7.3% of 
      the karyotypes and 0.5% had both numerical and structural abnormalities. Results 
      of FISH analyses yielded disomy frequencies of 0.10%, 0.11%, 0.04%, 0.05%, and 
      0.18% for chromosomes 1, 12, X, Y, and XY, respectively. The frequency of diploid 
      sperm was 0.09%. The frequency of abnormalities was not significantly increased 
      compared to control donors for any of the studies. Also, the frequencies of X- 
      and Y-bearing sperm did not differ significantly from 50% in the sperm 
      karyotyping or FISH studies.
FAU - Martin, R H
AU  - Martin RH
AD  - Department of Medical Genetics, Faculty of Medicine, University of Calgary, 
      Alberta, Canada.
FAU - Rademaker, A W
AU  - Rademaker AW
FAU - Leonard, N J
AU  - Leonard NJ
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 11056-06-7 (Bleomycin)
RN  - 5J49Q6B70F (Vincristine)
RN  - 80168379AG (Doxorubicin)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - Q573I9DVLP (Leucovorin)
RN  - VB0R961HZT (Prednisone)
RN  - YL5FZ2Y5U1 (Methotrexate)
RN  - MACOP-B protocol
SB  - IM
MH  - Aneuploidy
MH  - Antineoplastic Combined Chemotherapy Protocols/*therapeutic use
MH  - Bleomycin/therapeutic use
MH  - *Chromosome Aberrations
MH  - Cyclophosphamide/therapeutic use
MH  - Doxorubicin/therapeutic use
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leucovorin/therapeutic use
MH  - Lymphoma/*drug therapy
MH  - Male
MH  - Methotrexate/therapeutic use
MH  - Prednisone/therapeutic use
MH  - Spermatozoa/*ultrastructure
MH  - Vincristine/therapeutic use
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 0165460894001625 [pii]
AID - 10.1016/0165-4608(94)00162-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Mar;80(1):29-32. doi: 10.1016/0165-4608(94)00162-5.