PMID- 7540006
OWN - NLM
STAT- MEDLINE
DCOM- 19950710
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 211
IP  - 1
DP  - 1995 Jun 6
TI  - The cytomegalovirus US28 protein binds multiple CC chemokines with high affinity.
PG  - 325-30
AB  - Human cytomegalovirus encodes several proteins with high similarity to seven 
      transmembrane domain receptors. We investigated the ability of one of these 
      proteins, the product of the US28 open reading frame, to bind various 
      chemoattractant ligands. When transfected into COS-7 cells, the US28 product 
      conferred high affinity binding to the labeled chemokines monocyte 
      chemoattractant protein-1 (MCP-1) (Kd = 6.0 x 10(-10) M) and RANTES (Kd = 2.7 x 
      10(-10) M). Binding of these labeled ligands could be competed by the unlabeled 
      macrophage inflammatory proteins MIP-1 alpha and MIP-1 beta, with Kd values in 
      the range 1.2 x 10(-9) to 7.5 x 10(-9) M. Comparisons of the sequences of US28 
      and other receptors that bind chemokines should help to define regions 
      responsible for receptor-ligand interactions.
FAU - Kuhn, D E
AU  - Kuhn DE
AD  - Ohio State University, Neurobiotechnology Center, Columbus 43210, USA.
FAU - Beall, C J
AU  - Beall CJ
FAU - Kolattukudy, P E
AU  - Kolattukudy PE
LA  - eng
SI  - GENBANK/L20501
GR  - HL48916/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Lymphokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Monokines)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL4
MH  - Chemokine CCL5
MH  - Chemotactic Factors/*metabolism
MH  - Chlorocebus aethiops
MH  - Cytokines/*metabolism
MH  - Cytomegalovirus/*metabolism
MH  - DNA Primers
MH  - Kidney
MH  - Kinetics
MH  - Lymphokines/*metabolism
MH  - Macrophage Inflammatory Proteins
MH  - Molecular Sequence Data
MH  - Monocyte Chemoattractant Proteins
MH  - Monokines/*metabolism
MH  - Open Reading Frames
MH  - Protein Binding
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/*metabolism
MH  - Recombinant Proteins/biosynthesis/metabolism
MH  - Transfection
EDAT- 1995/06/06 00:00
MHDA- 1995/06/06 00:01
CRDT- 1995/06/06 00:00
PHST- 1995/06/06 00:00 [pubmed]
PHST- 1995/06/06 00:01 [medline]
PHST- 1995/06/06 00:00 [entrez]
AID - S0006-291X(85)71814-1 [pii]
AID - 10.1006/bbrc.1995.1814 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Jun 6;211(1):325-30. doi: 
      10.1006/bbrc.1995.1814.