PMID- 7542257
OWN - NLM
STAT- MEDLINE
DCOM- 19950822
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 108 ( Pt 4)
DP  - 1995 Apr
TI  - The role of the microtubular system in the cell response to HGF/SF.
PG  - 1659-67
AB  - The effects of the microtubular drugs colcemid and taxol on the morphological 
      changes induced by hepatocyte growth factor/scatter factor (HGF/SF) in MDCK cells 
      were studied. Dynamic changes in the area and shape of individual cells were 
      assessed by morphometric methods whereas alterations of the cytoskeleton were 
      assessed by immunomorphological methods. The results suggest that there are two 
      components in the response to HGF/SF: (a) activation of the extension of lamellae 
      leading to cell spreading; and (b) reorganization of microtubules leading to 
      polarization of cell shape. The latter response is highly sensitive to 
      microtubular drugs, especially taxol. HGF/SF induced spreading in taxol-treated 
      MDCK cells but these cells retained a non-polarized discoid shape and a pattern 
      of actin microfilament bundles characteristic of the untreated cells. Colcemid 
      and taxol did not prevent HGF/SF-induced migration of cells in Boyden chambers 
      but completely inhibited the outgrowth of multicellular strands and tubules from 
      cell aggregates in collagen gels. These results show that enhanced lamella 
      formation in response to HGF/SF without polarization of cell shape is sufficient 
      to induce cell motility. In contrast, microtubule-dependent polarization is 
      essential for complex morphogenetic responses such as tubulogenesis in collagen 
      gels.
FAU - Dugina, V B
AU  - Dugina VB
AD  - Belozersky Institute of Physical and Chemical Biology, Moscow State University, 
      Russia.
FAU - Alexandrova, A Y
AU  - Alexandrova AY
FAU - Lane, K
AU  - Lane K
FAU - Bulanova, E
AU  - Bulanova E
FAU - Vasiliev, J M
AU  - Vasiliev JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vimentin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 68238-35-7 (Keratins)
RN  - P88XT4IS4D (Paclitaxel)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement/drug effects/physiology
MH  - Cytoskeleton/drug effects/*ultrastructure
MH  - Demecolcine/pharmacology
MH  - Dogs
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Keratins/drug effects/ultrastructure
MH  - Kidney
MH  - Kinetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Microtubules/drug effects/*ultrastructure
MH  - Paclitaxel/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Vimentin/drug effects/ultrastructure
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1242/jcs.108.4.1659 [doi]
PST - ppublish
SO  - J Cell Sci. 1995 Apr;108 ( Pt 4):1659-67. doi: 10.1242/jcs.108.4.1659.