PMID- 7542367
OWN - NLM
STAT- MEDLINE
DCOM- 19950824
LR  - 20181130
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 70
IP  - 1
DP  - 1995
TI  - Human glomerular epithelial cells synthesize and secrete the third component of 
      complement.
PG  - 55-61
AB  - Complement proteins in serum are mainly synthesized by hepatocytes. Recently, 
      many cell types have been reported to synthesize complement in various tissues. 
      In this study, we report the synthesis and secretion of the third component of 
      complement (C3) by cultured glomerular epithelial cells (GEC). Using reverse 
      transcriptase polymerase reaction, we have found that GEC and whole kidney 
      expressed the C3 mRNA for C3. By ELISA, we have found that C3 was secreted in 
      culture supernatants harvested from cultured GEC. The secretion of C3 is 
      regulated by proinflammatory cytokines (IL-1 beta, TNF-alpha and IL-6). IL-1 beta 
      is shown to be the most potent stimulator of C3 secretion from GEC. The exact 
      significance of C3 produced at glomerular site is not clear, but its upregulation 
      by proinflammatory cytokines may suspect a role in local activation of complement 
      which may lead to glomerular injury. We further studied the expression of C3 step 
      regulatory proteins (membrane cofactor protein (MCP), decay-accelerating factor 
      (DAF), CR-1 and CD59 (a terminal step regulatory protein) by cultured GEC. 
      Treatment of GEC by proinflammatory cytokines IL-1 beta, TGF-beta, TNF-alpha and 
      IL-6 did not modify the expression of MCP, DAF and CR-1 whereas an increase in 
      the expression of CD59 could be observed after treatment with IL-1 beta and 
      TGF-beta. These results indicate that the expression of these regulatory proteins 
      is tissue specific and may be implicated in inflammatory processes.
FAU - Moutabarrik, A
AU  - Moutabarrik A
AD  - Department of Nephrology, Faculty of Medicine, Casablanca, Morocco.
FAU - Nakanishi, I
AU  - Nakanishi I
FAU - Matsumoto, M
AU  - Matsumoto M
FAU - Zaid, D
AU  - Zaid D
FAU - Seya, T
AU  - Seya T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Antigens, CD)
RN  - 0 (CD46 protein, human)
RN  - 0 (CD55 Antigens)
RN  - 0 (Complement C3)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antigens, CD/biosynthesis
MH  - Base Sequence
MH  - CD55 Antigens
MH  - Complement C3/*biosynthesis/drug effects/genetics
MH  - Cytokines/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelium/drug effects/metabolism
MH  - Flow Cytometry
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Kidney Glomerulus/drug effects/*metabolism/pathology
MH  - Membrane Cofactor Protein
MH  - Membrane Glycoproteins/biosynthesis
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/metabolism
MH  - RNA-Directed DNA Polymerase
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000188544 [doi]
PST - ppublish
SO  - Nephron. 1995;70(1):55-61. doi: 10.1159/000188544.