PMID- 7542411
OWN - NLM
STAT- MEDLINE
DCOM- 19950824
LR  - 20190830
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 46
IP  - 1-2
DP  - 1995 May
TI  - Dehydroepiandrosterone inhibits replication of feline immunodeficiency virus in 
      chronically infected cells.
PG  - 159-68
AB  - Dehydroepiandrosterone (DHEA) is a steroid hormone produced by the adrenal cortex 
      that serves as an intermediary in sex steroid synthesis. DHEA is produced in 
      abundance by humans and most other warm-blooded animals. Based upon previous 
      reports demonstrating the antiviral and immunostimulatory activities of DHEA and 
      DHEA-sulfate (DHEAS) we sought to determine whether introduction of these 
      compounds would affect replication of feline immunodeficiency virus (FIV) in 
      chronically infected cells. When cell number, cell viability, cellular DNA 
      synthesis, and levels of FIV reverse-transcriptase (RT) were measured in cell 
      cultures treated with various dilutions of DHEA or DHEAS it was found that the 
      production of FIV RT was inhibited by DHEA at levels where cellular viability and 
      DNA synthesis were not affected. At the concentrations tested DHEAS did not 
      inhibit FIV replication or impact on cellular viability or proliferation.
FAU - Bradley, W G
AU  - Bradley WG
AD  - All Children's Hospital, Laboratory of Retrovirology, St. Petersburg, FL 33701, 
      USA.
FAU - Kraus, L A
AU  - Kraus LA
FAU - Good, R A
AU  - Good RA
FAU - Day, N K
AU  - Day NK
LA  - eng
GR  - 1 T32 DA07245-04/DA/NIDA NIH HHS/United States
GR  - R01 CA40931-07/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (DNA, Viral)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Cats
MH  - Cell Count
MH  - Cell Division
MH  - Cell Line
MH  - Cell Survival
MH  - Cells, Cultured
MH  - DNA, Viral/biosynthesis
MH  - Dehydroepiandrosterone/*pharmacology
MH  - Immunodeficiency Virus, Feline/drug effects/*physiology
MH  - RNA-Directed DNA Polymerase/drug effects
MH  - T-Lymphocytes/*virology
MH  - Virus Replication/*drug effects
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 0165-2427(94)07015-Y [pii]
AID - 10.1016/0165-2427(94)07015-y [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1995 May;46(1-2):159-68. doi: 
      10.1016/0165-2427(94)07015-y.