PMID- 7543588
OWN - NLM
STAT- MEDLINE
DCOM- 19950914
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 9
DP  - 1995 Sep
TI  - The alpha-glucosidase inhibitor N-butyldeoxynojirimycin inhibits human 
      immunodeficiency virus entry at the level of post-CD4 binding.
PG  - 5791-7
AB  - The alpha-glucosidase inhibitor N-butyldeoxynojirimycin (NB-DNJ) is a potent 
      inhibitor of human immunodeficiency virus (HIV) replication and syncytium 
      formation in vitro. However, the exact mechanism of action of NB-DNJ remains to 
      be determined. In this study we have examined the impairment of HIV infectivity 
      mediated by NB-DNJ. By two independent HIV entry assays [PCR-based HIV entry 
      assay and entry of Cocal(HIV) pseudotypes], the reduction in infectivity was 
      found to be due to an impairment of viral entry. No effect of NB-DNJ treatment 
      was seen on the kinetics of the interaction between gp120 and CD4 (surface 
      plasmon resonance; BIAcore) or on the binding of virus particles to H9 cells 
      (using radiolabeled virions). We therefore conclude that a major mechanism of 
      action of NB-DNJ as an inhibitor of HIV replication is the impairment of viral 
      entry at the level of post-CD4 binding, due to an effect on viral envelope 
      components.
FAU - Fischer, P B
AU  - Fischer PB
AD  - Department of Biochemistry, University of Oxford, United Kingdom.
FAU - Collin, M
AU  - Collin M
FAU - Karlsson, G B
AU  - Karlsson GB
FAU - James, W
AU  - James W
FAU - Butters, T D
AU  - Butters TD
FAU - Davis, S J
AU  - Davis SJ
FAU - Gordon, S
AU  - Gordon S
FAU - Dwek, R A
AU  - Dwek RA
FAU - Platt, F M
AU  - Platt FM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antiviral Agents)
RN  - 0 (CD4 Antigens)
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Receptors, Virus)
RN  - 19130-96-2 (1-Deoxynojirimycin)
RN  - ADN3S497AZ (miglustat)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - 1-Deoxynojirimycin/*analogs & derivatives/pharmacology
MH  - Antiviral Agents/*pharmacology
MH  - CD4 Antigens/*physiology
MH  - Cell Line
MH  - *Glycoside Hydrolase Inhibitors
MH  - HIV Envelope Protein gp120/metabolism
MH  - HIV Reverse Transcriptase
MH  - HIV-1/drug effects/*pathogenicity/*physiology
MH  - Humans
MH  - Kinetics
MH  - RNA-Directed DNA Polymerase/analysis
MH  - Receptors, Virus/physiology
MH  - Time Factors
MH  - Virion/drug effects/pathogenicity/physiology
MH  - Virus Replication/*drug effects
PMC - PMC189444
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
PMCR- 1995/09/01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
PHST- 1995/09/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.9.5791-5797.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Sep;69(9):5791-7. doi: 10.1128/JVI.69.9.5791-5797.1995.