PMID- 7544300
OWN - NLM
STAT- MEDLINE
DCOM- 19950925
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 370
IP  - 1-2
DP  - 1995 Aug 14
TI  - Effect of glucagon on insulin receptor substrate-1 (IRS-1) phosphorylation and 
      association with phosphatidylinositol 3-kinase (PI 3-kinase).
PG  - 131-4
AB  - In the present study we have examined the levels and phosphorylation state of the 
      insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the 
      association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the 
      liver and muscle of rats treated with glucagon. There was a decrease in the 
      insulin-stimulated receptor and IRS-1 phosphorylation levels which was paralleled 
      by a reduced association between IRS-1 and PI 3-kinase in vivo in the liver and 
      muscle of glucagon-treated rats. These observations suggest that glucagon, 
      probably acting through cAMP, may impair insulin signaling in the three early 
      steps in insulin action after binding.
FAU - Saad, M J
AU  - Saad MJ
AD  - Department of Internal Medicine, FCM, UNICAMP, Cidade Universitaria Zeferino Vaz, 
      Campinas, SP, Brazil.
FAU - Hartmann, L G
AU  - Hartmann LG
FAU - de Carvalho, D S
AU  - de Carvalho DS
FAU - Galoro, C A
AU  - Galoro CA
FAU - Brenelli, S L
AU  - Brenelli SL
FAU - Carvalho, C R
AU  - Carvalho CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, rat)
RN  - 0 (Phosphoproteins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - 9007-92-5 (Glucagon)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
SB  - IM
MH  - Animals
MH  - Glucagon/*pharmacology
MH  - Insulin/*pharmacology
MH  - Insulin Receptor Substrate Proteins
MH  - Liver/drug effects/*metabolism
MH  - Male
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Phosphatidylinositol 3-Kinases
MH  - Phosphoproteins/drug effects/isolation & purification/*metabolism
MH  - Phosphorylation
MH  - Phosphotransferases (Alcohol Group Acceptor)/drug effects/*metabolism
MH  - Phosphotyrosine
MH  - Rats
MH  - Reference Values
MH  - Tyrosine/analogs & derivatives/metabolism
EDAT- 1995/08/14 00:00
MHDA- 1995/08/14 00:01
CRDT- 1995/08/14 00:00
PHST- 1995/08/14 00:00 [pubmed]
PHST- 1995/08/14 00:01 [medline]
PHST- 1995/08/14 00:00 [entrez]
AID - 0014-5793(95)00809-N [pii]
AID - 10.1016/0014-5793(95)00809-n [doi]
PST - ppublish
SO  - FEBS Lett. 1995 Aug 14;370(1-2):131-4. doi: 10.1016/0014-5793(95)00809-n.