PMID- 7544878
OWN - NLM
STAT- MEDLINE
DCOM- 19951004
LR  - 20180214
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 61
IP  - 6
DP  - 1995 Jun
TI  - Galanin-binding sites in the female rat brain are regulated across puberty yet 
      similar to the male pattern in adulthood.
PG  - 646-54
AB  - The neuropeptide galanin (GAL) has been implicated in a variety of neuroendocrine 
      functions and has been shown to be regulated by gonadal hormones in several brain 
      regions. We have used slice binding and quantitative autoradiography techniques 
      to determine whether the activation of GAL pathways across puberty in female rats 
      is associated with changes in the density of GAL binding in telencephalic and 
      diencephalic regions as we previously observed in male rats. We have also asked 
      whether sex differences in GAL immunoreactivity and GAL gene expression detected 
      in some brain regions would be paralleled by sex differences in 125I-GAL-binding 
      density in adult male and female rat brains. To control for intrinsic differences 
      in the level of endogenous GAL synthesis and release, brain slices from 
      prepubertal female and adult male and female rats were treated with guanosine 
      5'-triphosphate (GTP) to induce dissociation of endogenous GAL from its binding 
      sites prior to incubation with radiolabeled ligand. 125I-GAL binding was 
      significantly reduced in seven brain regions of adult compared with prepubertal 
      female rats. These regions included the islands of Calleja (p < or = 0.03), the 
      medial amygdaloid nucleus, posterodorsal division (p < or = 0.05), median 
      eminence (p < or = 0.02), medial habenular nucleus (p < or = 0.05), rhomboid 
      thalamic nucleus (p < or = 0.05), and paraventricular (p < or = 0.05) and 
      intermediodorsal (p < or = 0.02) thalamic nuclei. Only one region, the lateral 
      preoptic area, exhibited significantly enhanced 125I-GAL binding in adult female 
      (p < or = 0.04) compared with prepubertal animals.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Planas, B
AU  - Planas B
AD  - Department of Psychiatry and Behavioral Sciences, University of Washington, 
      Seattle 98195, USA.
FAU - Kolb, P E
AU  - Kolb PE
FAU - Raskind, M A
AU  - Raskind MA
FAU - Miller, M A
AU  - Miller MA
LA  - eng
GR  - AG05135/AG/NIA NIH HHS/United States
GR  - AG08419/AG/NIA NIH HHS/United States
GR  - AG10917/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Galanin)
RN  - 0 (Receptors, Gastrointestinal Hormone)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 88813-36-9 (Galanin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Brain/anatomy & histology
MH  - Brain Chemistry/*physiology
MH  - Estradiol/blood
MH  - Female
MH  - GTP-Binding Proteins/metabolism
MH  - Galanin
MH  - In Vitro Techniques
MH  - Iodine Radioisotopes
MH  - Male
MH  - Peptides/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Galanin
MH  - Receptors, Gastrointestinal Hormone/*metabolism
MH  - Sex Characteristics
MH  - Sexual Maturation/*physiology
MH  - Testosterone/blood
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1159/000126891 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1995 Jun;61(6):646-54. doi: 10.1159/000126891.