PMID- 7545470
OWN - NLM
STAT- MEDLINE
DCOM- 19951019
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 7
DP  - 1995 Oct 1
TI  - Production of chemokines, interleukin-8 and monocyte chemoattractant protein-1, 
      during monocyte: endothelial cell interactions.
PG  - 2767-73
AB  - The extravasation of leukocytes from the lumen of the vessel to a site of 
      inflammation requires specific binding events. The interaction of leukocytes with 
      endothelium, via specific receptors, may provide intracellular signals that 
      activate extravasating cells. In the present study, we have investigated the 
      production of chemokines, interleukin-8 (IL-8), and monocyte chemoattractant 
      protein-1 (MCP-1) during monocyte: endothelial cell interactions. Both 
      unstimulated and interferon-gamma (IFN-gamma)-prestimulated human umbilical vein 
      endothelial cells (HUVEC) produced low constitutive levels of IL-8 and MCP-1. The 
      addition of enriched monocytes with unstimulated HUVEC resulted in synergistic 
      increases in production of both IL-8 and MCP-1. Monocytes cultured with 
      IFN-gamma-preactivated HUVECs demonstrated little additional increase in IL-8 and 
      MCP-1 production in coculture assays compared with unstimulated HUVEC. Northern 
      blot analysis paralleled the protein data, demonstrating upregulated expression 
      of IL-8 and MCP-1 mRNA in stimulated and unstimulated coculture assays. Culture 
      of enriched monocytes and endothelial cells in transwells demonstrated no 
      increases in IL-8 or MCP-1, indicating the necessity for cellular contact for 
      chemokine production. In previous investigations, we have demonstrated that 
      increased monocyte-derived MIP-1 alpha production was induced by intracellular 
      adhesion molecule-1 (ICAM-1) interactions on activated HUVECs. In contrast, 
      addition of anti-ICAM-1 monoclonal antibodies (MoAbs) did not diminish the 
      production of IL-8 and MCP-1 in the present study. Furthermore, neither 
      antibodies to IL-1 nor tumor necrosis factor (TNF) diminished the production of 
      either IL-8 or MCP-1. However, when soluble matrix proteins were added to the 
      coculture to block cellular interactions, the chemokine protein and mRNA levels 
      were significantly decreased. IL-8 production was decreased by both soluble 
      collagen and fibronectin, whereas MCP-1 was decreased by only soluble collagen, 
      suggesting differential activation pathways. These results indicate that IL-8 and 
      MCP-1 production are increased during monocyte and endothelial cell interactions 
      in part due to matrix protein binding mechanisms. This mechanism may serve a role 
      in cell activation, production of chemokines, as well as extravasation and 
      recruitment of additional leukocytes during inflammatory responses.
FAU - Lukacs, N W
AU  - Lukacs NW
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0602, USA.
FAU - Strieter, R M
AU  - Strieter RM
FAU - Elner, V
AU  - Elner V
FAU - Evanoff, H L
AU  - Evanoff HL
FAU - Burdick, M D
AU  - Burdick MD
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - HL02401/HL/NHLBI NIH HHS/United States
GR  - HL31963/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Antibodies, Monoclonal/pharmacology
MH  - Cell Adhesion Molecules/immunology/physiology
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis/genetics
MH  - Collagen/pharmacology
MH  - Cytokines/*biosynthesis
MH  - Endothelium, Vascular/*cytology/metabolism
MH  - Fibronectins/pharmacology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/immunology/physiology
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-8/*biosynthesis/genetics
MH  - Kinetics
MH  - Monocytes/*cytology/metabolism
MH  - RNA, Messenger/metabolism
MH  - Umbilical Veins
MH  - Vascular Cell Adhesion Molecule-1
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - S0006-4971(20)68735-3 [pii]
PST - ppublish
SO  - Blood. 1995 Oct 1;86(7):2767-73.