PMID- 7545567
OWN - NLM
STAT- MEDLINE
DCOM- 19951013
LR  - 20190830
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 14
IP  - 6
DP  - 1995 Jun
TI  - SV40-immortalized and primary cultured human retinal pigment epithelial cells 
      share similar patterns of cytokine-receptor expression and cytokine 
      responsiveness.
PG  - 495-503
AB  - Retinal pigment epithelial (RPE) cells produce and respond to a variety of 
      cytokines; however, molecular and biochemical studies are restricted by the 
      limited access to large numbers of pure cells and the variability associated with 
      different donor sources. Despite success in establishing primary human RPE (HRPE) 
      cell cultures, the inability to sustain consistent proliferation rates and 
      morphology over several passages remains a concern. This problem was approached 
      by using an immortalized line of simian virus (SV)40 transformed fetal HRPE cells 
      (SVRPE). Cytokine production, receptor expression and responsiveness in the SVRPE 
      cell line was analyzed to determine the usefulness of this model for studying 
      HRPE-cytokine interactions. Using reverse transcriptase polymerase chain reaction 
      (RT-PCR), HRPE and SVRPE cells demonstrated an identical pattern of interleukin-1 
      receptor (IL-1R), IL-2R (alpha sub-unit), IL-6R, interferon (IFN)-gamma R and 
      tumor necrosis factor-alpha (TNF)R p55 expression. No amplification products for 
      TNFR p75 or granulocyte/macrophage colony stimulating factor (GM-CSF)R were 
      demonstrated in either population. IFN-gamma stimulation induced surface human 
      leukocyte antigen (HLA)-DR in both SVRPE and HRPE, while TNF treatment induced 
      surface expression of intercellular adhesion molecule (ICAM)-1 on SVRPE and 
      upregulated ICAM from basal levels on HRPE. Both cell types showed amplification 
      products for interleukin (IL)-1 beta, IL-6 and transforming growth factor 
      (TGF)-beta 1 using RT-PCR. The bioassays demonstrated that both populations of 
      unstimulated cells constitutively secrete very low levels of TGF-beta and no 
      IL-6.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Sippy, B D
AU  - Sippy BD
AD  - Department of Pathology, University of Southern California School of Medicine, 
      Los Angeles 90033, USA.
FAU - Hofman, F M
AU  - Hofman FM
FAU - He, S
AU  - He S
FAU - Osusky, R
AU  - Osusky R
FAU - Sheu, S J
AU  - Sheu SJ
FAU - Walker, S M
AU  - Walker SM
FAU - Ryan, S J
AU  - Ryan SJ
FAU - Hinton, D R
AU  - Hinton DR
LA  - eng
GR  - R01-EY02061/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Cytokines)
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytokine)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Base Sequence
MH  - Cell Division
MH  - Cell Line, Transformed
MH  - Cell Transformation, Viral/physiology
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis/*pharmacology
MH  - DNA Probes/chemistry
MH  - Flow Cytometry
MH  - Humans
MH  - Molecular Sequence Data
MH  - Pigment Epithelium of Eye/cytology/drug effects/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - RNA-Directed DNA Polymerase
MH  - Receptors, Cytokine/*biosynthesis
MH  - Simian virus 40/physiology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.3109/02713689509003761 [doi]
PST - ppublish
SO  - Curr Eye Res. 1995 Jun;14(6):495-503. doi: 10.3109/02713689509003761.