PMID- 7546335
OWN - NLM
STAT- MEDLINE
DCOM- 19951109
LR  - 20190913
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 12
IP  - 4
DP  - 1995 Jul-Aug
TI  - Glutamate and N-methyl-D-aspartate binding sites in the guinea pig hippocampus: 
      ontogeny and effect of acute in vitro ethanol exposure.
PG  - 369-75
AB  - The objectives of this study were to characterize the ontogeny of the L-glutamate 
      (glutamate) and N-methyl-D-aspartate (NMDA) binding sites in the developing 
      guinea pig hippocampus, and to determine the effect of acute in vitro ethanol 
      exposure on these binding sites. Specific [3H]glutamate binding and 
      NMDA-sensitive [3H]glutamate binding were determined using a guinea pig 
      hippocampal synaptic membrane preparation (HSMP). To characterize the ontogeny of 
      the density (Bmax) and affinity (Kd) of the glutamate and NMDA binding sites, 
      saturation analysis was conducted on HSMP of guinea pigs at gestational day (GD) 
      50 (immature fetus; term, GD 68), GD 62 (mature, near-term fetus), postnatal day 
      (PD) 13 (neonate), and PD > 60 (adult). To examine the effect of ethanol on the 
      glutamate and NMDA binding sites, HSMP of guinea pigs at GD 50, GD 62, PD 13, and 
      PD > 60 was incubated with ethanol (0-100 mM), followed by determination of 
      specific [3H]glutamate binding and NMDA-sensitive [3H]glutamate binding. To 
      determine the effect of 50 mM ethanol on the Bmax and Kd of the glutamate and 
      NMDA binding sites, HSMP of guinea pigs at GD 62 and PD > 60 was incubated with 0 
      or 50 mM ethanol followed by saturation analysis. The Bmax values of the 
      hippocampal glutamate and NMDA binding sites were greater at GD 62 and PD 13 
      compared with GD 50 and PD > 60, but there was no change in the Kd of the binding 
      sites throughout development.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Abdollah, S
AU  - Abdollah S
AD  - Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 3K9958V90M (Ethanol)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Binding Sites/drug effects
MH  - Embryonic and Fetal Development
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Fetus/*metabolism
MH  - Glutamic Acid/*metabolism
MH  - Guinea Pigs/embryology
MH  - Hippocampus/embryology/*metabolism
MH  - Male
MH  - N-Methylaspartate/*metabolism
MH  - Synaptic Membranes/metabolism
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 0741-8329(95)00020-R [pii]
AID - 10.1016/0741-8329(95)00020-r [doi]
PST - ppublish
SO  - Alcohol. 1995 Jul-Aug;12(4):369-75. doi: 10.1016/0741-8329(95)00020-r.