PMID- 7546336
OWN - NLM
STAT- MEDLINE
DCOM- 19951109
LR  - 20190913
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 12
IP  - 4
DP  - 1995 Jul-Aug
TI  - Effect of chronic maternal ethanol administration on glutamate and 
      N-methyl-D-aspartate binding sites in the hippocampus of the near-term fetal 
      guinea pig.
PG  - 377-82
AB  - The objective of this study was to determine the effect of chronic maternal 
      administration of ethanol on hippocampal L-glutamate (glutamate) and 
      N-methyl-D-aspartate (NMDA) binding sites in the near-term fetal guinea pig. 
      Starting on gestational day (GD) 2, pregnant guinea pigs received one of the 
      following oral treatments up to and including GD 62 (term, about GD 68): 4 g 
      ethanol.kg maternal body weight-1.day-1; isocaloric sucrose and pair-feeding; or 
      water. Maternal blood ethanol concentration was determined at 1 h after the daily 
      ethanol dose on GD 59. Fetuses were studied at GD 63 (mature, near-term fetus). 
      Fetal body weight and brain weight were determined. The density (Bmax) and 
      affinity (Kd) of the glutamate and NMDA binding sites in the fetal hippocampus 
      were measured using a radioligand membrane binding assay; saturation analysis was 
      conducted on hippocampal synaptic membrane preparation (HSMP). Maternal blood 
      ethanol concentration on GD 59 was 269 +/- 111 (SD) mg/dl (59 +/- 24 mM). There 
      was no maternal or embryonic fetal lethality in any of the three treatment 
      groups, and ethanol treatment did not affect maternal body weight gain compared 
      with sucrose or water treatment. Fetal brain weight, but not body weight, was 
      decreased in the ethanol treatment group compared with the sucrose and water 
      treatment groups. The Bmax values of the glutamate and NMDA binding sites were 
      decreased in the ethanol treatment group compared with the sucrose and water 
      treatment groups; there was no difference in the Kd values of the glutamate and 
      NMDA binding sites among the three treatment groups.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Abdollah, S
AU  - Abdollah S
AD  - Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 3K9958V90M (Ethanol)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
EIN - Alcohol 1996 Jan-Feb;13(1):107
MH  - Animals
MH  - Binding Sites/drug effects
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Gestational Age
MH  - Glutamic Acid/*metabolism
MH  - Guinea Pigs
MH  - Hippocampus/*metabolism
MH  - Humans
MH  - N-Methylaspartate/*metabolism
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Synaptic Membranes/metabolism
MH  - Time Factors
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 0741-8329(95)00021-I [pii]
AID - 10.1016/0741-8329(95)00021-i [doi]
PST - ppublish
SO  - Alcohol. 1995 Jul-Aug;12(4):377-82. doi: 10.1016/0741-8329(95)00021-i.