PMID- 7550383
OWN - NLM
STAT- MEDLINE
DCOM- 19951103
LR  - 20191031
IS  - 0724-4983 (Print)
IS  - 0724-4983 (Linking)
VI  - 13
IP  - 3
DP  - 1995
TI  - Molecular cytogenetics: unraveling of the genetic composition of individual cells 
      by fluorescence in situ hybridization and digital imaging microscopy.
PG  - 138-42
AB  - Molecular biology techniques allow the unraveling of the genetic alterations that 
      cause or accompany malignant disease. Since tumors are often heterogeneous, 
      biochemical analysis of tissue homogenates is of limited diagnostic value. This 
      paper gives examples of methods that are presently operational to analyze the 
      genetic composition of individual cells. They are based on fluorescence in situ 
      hybridization (FISH) and digital imaging microscopy. First, the current status of 
      indirect and direct FISH staining methods with respect to probe labeling, 
      detection sensitivity, multiplicity, and DNA resolution is summarized. Microscope 
      hardware as well as charge-coupled device (CCD) cameras required for FISH 
      analysis are then described. Applications potentially important for the analysis 
      of urological malignancies, such as the automated enumeration of chromosomal 
      abnormalities (counting of dots in interphase cells) and high-resolution DNA 
      mapping on highly extended chromatin, are described in detail. Finally, the 
      limitations of the present methodology and its future prospects are discussed.
FAU - Tanke, H J
AU  - Tanke HJ
AD  - Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.
FAU - Florijn, R J
AU  - Florijn RJ
FAU - Vrolijk, J
AU  - Vrolijk J
FAU - Raap, A K
AU  - Raap AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Germany
TA  - World J Urol
JT  - World journal of urology
JID - 8307716
SB  - IM
MH  - Chromosome Mapping
MH  - Cytogenetics/instrumentation/*methods
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Microscopy, Fluorescence
MH  - Neoplasms/*genetics
MH  - *Signal Processing, Computer-Assisted
RF  - 19
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF00184867 [doi]
PST - ppublish
SO  - World J Urol. 1995;13(3):138-42. doi: 10.1007/BF00184867.