PMID- 7551546
OWN - NLM
STAT- MEDLINE
DCOM- 19951103
LR  - 20191031
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 5
DP  - 1995 Aug
TI  - Barley telomeres are associated with two different types of satellite DNA 
      sequences.
PG  - 315-20
AB  - The genomic organization of two different types of satellite DNA sequences was 
      analysed by means of fluorescence in situ hybridization (FISH) and pulsed-field 
      gel electrophoresis (PFGE) in barley. Satellite HvT01 was detected at all 
      chromosome ends except the long arms of chromosomes 2 and 7. The unrelated 
      satellite pAS1 was found at all chromosome ends except the long arm of chromosome 
      7 and at two interstitial sites, both located on the long arm of chromosome 4 on 
      the standard karyotype. Southern and in situ hybridization further indicate that 
      pAS1 also occurs interspersed in the barley genome. For most chromosome ends, the 
      linear order of HvT01 and pAS1 could not be determined by in situ hybridization 
      except at the short arms of chromosomes 2 and 6, where HvT01 is more distal than 
      pAS1. This is confirmed by PFGE analysis, HvT01 being frequently associated with 
      the telomeric repeat but not pAS1. Furthermore, we found that HvT01 occurred in 
      clusters up to 1000 kb in size, whereas the pAS1 cluster had a maximum size of 
      500 kb. Sequence comparison revealed that both satellites are completely 
      unrelated and differ considerably in their G + C contents.
FAU - Brandes, A
AU  - Brandes A
AD  - Department of Cell Biology, John Innes Centre for Plant Science Research, 
      Norwich, UK.
FAU - Roder, M S
AU  - Roder MS
FAU - Ganal, M W
AU  - Ganal MW
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Plant/*genetics
MH  - DNA, Satellite/*genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Sequence Homology, Nucleic Acid
MH  - *Telomere
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1007/BF00713070 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Aug;3(5):315-20. doi: 10.1007/BF00713070.