PMID- 7551549
OWN - NLM
STAT- MEDLINE
DCOM- 19951102
LR  - 20231213
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 6
DP  - 1995 Sep
TI  - Chromosomal location of endogenous geminivirus-related DNA sequences in Nicotiana 
      tabacum L.
PG  - 346-50
AB  - The N. tabacum (tobacco) nuclear genome carries approximately 25 multiple direct 
      repeats of a geminivirus-related DNA (GRD) sequence that probably arose by 
      illegitimate recombination, following geminivirus infection, during Nicotiana 
      evolution. Each GRD repeat carries sequences similar to the geminiviral AL1 gene 
      of the tomato golden mosaic virus (TGMV), encoding a protein required for viral 
      DNA replication, plus the cis-essential replication origin. Using a cloned 14-kb 
      GRD repeat sequence as a probe for fluorescence in situ hybridization (FISH), we 
      identified a unique tobacco chromosome carrying GRD. Translocations between 
      chromosomes of the tobacco S and T genomes were used as physical markers by 
      sequentially hybridizing chromosomes with labelled GRD and total genomic DNA from 
      N. sylvestris (equivalent to the S genome). The 25S, 18S and 5.8S ribosomal gene 
      clusters were detected in double-labelling experiments for use as additional 
      markers to identify the chromosomal location of GRD. GRD occupies one site on a 
      homologous pair of small submetacentrics from the T genome characterized by a 
      lack of either translocated segments from the S genome or ribosomal genes. GRD 
      provides an additional marker for the small chromosomes of the T genome and a 
      useful phylogenetic tool.
FAU - Kenton, A
AU  - Kenton A
AD  - Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, UK.
FAU - Khashoggi, A
AU  - Khashoggi A
FAU - Parokonny, A
AU  - Parokonny A
FAU - Bennett, M D
AU  - Bennett MD
FAU - Lichtenstein, C
AU  - Lichtenstein C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes/*genetics
MH  - DNA Probes
MH  - DNA, Viral/*analysis
MH  - Geminiviridae/*genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis
MH  - Mitosis
MH  - *Plants, Toxic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Nicotiana/*genetics/virology
MH  - Translocation, Genetic
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1007/BF00710015 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Sep;3(6):346-50. doi: 10.1007/BF00710015.