PMID- 7552476
OWN - NLM
STAT- MEDLINE
DCOM- 19951024
LR  - 20191023
IS  - 1077-9450 (Print)
IS  - 1077-9450 (Linking)
VI  - 10
IP  - 2
DP  - 1995 Oct 1
TI  - Effects of viral virulence on intrauterine growth in SIV-infected fetal rhesus 
      macaques (Macaca mulatta).
PG  - 129-38
AB  - Studies with a simian immunodeficiency virus (SIV)-infected fetal monkey model 
      were conducted with a focus on fetal growth and viral pathogenesis. Twenty-six 
      fetuses were inoculated in utero via ultrasound guidance with an uncloned 
      pathogenic strain of SIV or vehicle during the second or third trimesters 
      [gestational day (GD) 65, 110, or 130], sonographically monitored weekly 
      (biometrics, blood flow), then necropsied at incremental time points 
      postinfection. Peripheral blood hematologic (complete blood counts, clinical 
      chemistries), immunologic (immunophenotyping), and endocrine studies 
      [insulin-like growth factor (IGF), IGF-binding proteins (IGFBP)] were conducted. 
      Severe intrauterine growth restriction (IUGR), oligohydramnios, and altered 
      lymphocyte counts were noted for fetuses infected on GD 65. Less severe effects 
      were detected for fetuses inoculated at the later time points, with severity 
      dependent upon the length of SIV infection in utero. IGF studies indicated 
      significant reductions in IGF-I and elevated immunoreactive levels of IGFBP-3 in 
      infected fetuses during the third trimester. Parallel studies conducted with four 
      fetuses infected on GD 65 with a nonpathogenic, molecularly cloned virus 
      (SIVmac1A11) resulted in normal fetal growth, with no effects on hematopoiesis or 
      IGF/IGFBP levels, and no evidence of clinical disease. Taken together, these 
      studies show that (1) infection of fetuses during the early second trimester with 
      an uncloned pathogenic strain of SIV results in severe IUGR and a disruption in 
      the molar ratio of IGF:IGFBP-3, and (2) outcome of fetal SIV infection is 
      determined by the timing of infection and the virulence of the viral inoculum.
FAU - Tarantal, A F
AU  - Tarantal AF
AD  - California Regional Primate Research Center, University of California, Davis 
      95616-8542, USA.
FAU - Marthas, M L
AU  - Marthas ML
FAU - Gargosky, S E
AU  - Gargosky SE
FAU - Otysula, M
AU  - Otysula M
FAU - McChesney, M B
AU  - McChesney MB
FAU - Miller, C J
AU  - Miller CJ
FAU - Hendrickx, A G
AU  - Hendrickx AG
LA  - eng
GR  - RR00169/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Acquir Immune Defic Syndr Hum Retrovirol
JT  - Journal of acquired immune deficiency syndromes and human retrovirology : 
      official publication of the International Retrovirology Association
JID - 9501482
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.99.- (insulin-like growth factor binding protein-3 protease)
SB  - IM
MH  - Animals
MH  - CD4-CD8 Ratio
MH  - *Embryonic and Fetal Development
MH  - Endopeptidases/blood
MH  - Female
MH  - Fetal Blood/metabolism
MH  - Fetal Growth Retardation/blood/immunology/*virology
MH  - Fetal Monitoring
MH  - Gestational Age
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Lymphocytes/virology
MH  - Macaca mulatta
MH  - Male
MH  - Oligohydramnios/blood/immunology/*virology
MH  - Pregnancy
MH  - Simian Acquired Immunodeficiency Syndrome/blood/*etiology/immunology
MH  - Simian Immunodeficiency Virus/isolation & purification/*pathogenicity
MH  - Virulence
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1097/00042560-199510020-00004 [doi]
PST - ppublish
SO  - J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Oct 1;10(2):129-38. doi: 
      10.1097/00042560-199510020-00004.