PMID- 7553377
OWN - NLM
STAT- MEDLINE
DCOM- 19951025
LR  - 20191031
IS  - 1354-523X (Print)
IS  - 1354-523X (Linking)
VI  - 1
IP  - 1
DP  - 1995 Mar
TI  - Comparison of the osteolytic activity of surface-associated proteins of bacteria 
      implicated in periodontal disease.
PG  - 26-31
AB  - OBJECTIVES: To compare the osteolytic activity of surface-associated material 
      (SAM) and lipid A-associated proteins (LAPs) from periodontopathogenic bacteria. 
      MATERIALS AND METHODS: Surface-associated material was extracted from the surface 
      and LAPs from the cell walls of a range of periodontopathic bacteria including 
      Actinobacillus actinomycetemcomitans and Eikenella corrodens. These bacterial 
      fractions were assayed to determine their composition and their capacity to 
      induce bone resorption was determined by use of the neonatal murine calvarial 
      bone resorption assay. RESULTS: The SAMs from E. corrodens and A. 
      actinomycetemcomitans demonstrated bone-resorbing capacity at concentrations as 
      low as 1 ng ml-1 which, given the molecular weights of the active components, is 
      in the picomolar range of activity. In contrast, the SAMs from the other three 
      bacteria were significantly less potent and showed a lower efficacy. The LAPs all 
      showed significant, and similar, capacities to induce bone breakdown. 
      CONCLUSIONS: This is the first demonstration that LAP from periodontopathic 
      bacteria can stimulate bone degradation. The LAPs from diverse bacteria all 
      produced similar levels of bone-resorbing activity. In contrast, the SAM showed 
      significant differences in potency and in efficacy (maximal stimulation). This 
      may mean that in vivo certain periodontopathic bacteria have significantly more 
      bone-resorbing capacity than others and should be therapeutic targets.
FAU - Reddi, K
AU  - Reddi K
AD  - Maxillofacial Surgery Research Unit, Eastman Dental Institute for Oral Health 
      Care Sciences, University of London, United Kingdom.
FAU - Meghji, S
AU  - Meghji S
FAU - Wilson, M
AU  - Wilson M
FAU - Henderson, B
AU  - Henderson B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Endotoxins)
RN  - 0 (Lipid A)
RN  - 0 (lipid A-associated protein, Bacteria)
MH  - Aggregatibacter actinomycetemcomitans/chemistry/pathogenicity/physiology
MH  - Alveolar Bone Loss/*microbiology
MH  - Animals
MH  - Bacterial Outer Membrane Proteins/*physiology
MH  - Bacterial Proteins/physiology
MH  - Campylobacter/chemistry/pathogenicity/physiology
MH  - Eikenella corrodens/chemistry/pathogenicity/physiology
MH  - Endotoxins/analysis/physiology
MH  - Fimbriae, Bacterial/chemistry
MH  - Gram-Negative Anaerobic Bacteria/chemistry/*pathogenicity/physiology
MH  - Lipid A/physiology
MH  - Mice
MH  - Osteolysis/chemically induced/*microbiology
MH  - Porphyromonas gingivalis/chemistry/pathogenicity/physiology
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1111/j.1601-0825.1995.tb00153.x [doi]
PST - ppublish
SO  - Oral Dis. 1995 Mar;1(1):26-31. doi: 10.1111/j.1601-0825.1995.tb00153.x.