PMID- 7553779
OWN - NLM
STAT- MEDLINE
DCOM- 19951103
LR  - 20191210
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 17
IP  - 1
DP  - 1995 Jan
TI  - Staurosporine enhances Ca2+ entry induced by depletion of intracellular Ca2+ 
      stores in rat parotid acinar cells.
PG  - 32-40
AB  - The effect of staurosporine on the Ca2+ signalling induced by the muscarinic 
      receptor agonist carbachol (CCh) was studied in Fura-2-loaded rat parotid acinar 
      cells. At concentrations > 1 nM, staurosporine dose-dependently enhanced the 
      sustained increase in cytosolic free Ca2+ concentration ([Ca2+]i), but did not 
      affect the peak [Ca2+]i seen just after stimulation. The enhancement of the 
      sustained increase in [Ca2+]i was not attenuated by the protein kinase C 
      activator, 4 beta-phorbol 12-myristate 13-acetate, and not mimicked by another 
      inhibitor of protein kinase C, K-252a, suggesting that the effect of 
      staurosporine on the CCh-induced Ca2+ signalling may be due to a mechanism 
      independent of the inhibitory action on protein kinase C. Staurosporine also 
      enhanced the increases in [Ca2+]i induced by the microsomal Ca(2+)-ATPase 
      inhibitor thapsigargin (TG) and the Ca2+ ionophore ionomycin (Iono). When the 
      cells were stimulated by CCh, TG, or Iono in the absence of extracellular Ca2+, a 
      transient increase in [Ca2+]i due to Ca2+ release from intracellular stores was 
      observed. This increase in [Ca2+]i was unaffected by preincubation with 
      staurosporine. However, when Ca2+ was added to the extracellular medium after 
      [Ca2+]i had returned to the resting level, the increase in [Ca2+]i was 
      significantly enhanced by staurosporine. In addition, staurosporine accelerated 
      the Mn2+ influx following the addition of CCh, TG, or Iono. These results suggest 
      that staurosporine modulates the Ca2+ entry system activated by depletion of 
      intracellular Ca2+ stores in rat parotid acinar cells.
FAU - Tojyo, Y
AU  - Tojyo Y
AD  - Department of Dental Pharmacology, School of Dentistry, Health Sciences 
      University of Hokkaido, Japan.
FAU - Tanimura, A
AU  - Tanimura A
FAU - Matsumoto, Y
AU  - Matsumoto Y
FAU - Sugiya, H
AU  - Sugiya H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Alkaloids)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ionophores)
RN  - 0 (Muscarinic Agonists)
RN  - 0 (Terpenes)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 56092-81-0 (Ionomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - H88EPA0A3N (Staurosporine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Carbachol/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - In Vitro Techniques
MH  - Intracellular Fluid/drug effects/metabolism
MH  - Ion Transport/drug effects
MH  - Ionomycin/pharmacology
MH  - Ionophores/pharmacology
MH  - Male
MH  - Manganese/metabolism
MH  - Muscarinic Agonists/pharmacology
MH  - Parotid Gland/cytology/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Staurosporine
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 0143-4160(95)90100-0 [pii]
AID - 10.1016/0143-4160(95)90100-0 [doi]
PST - ppublish
SO  - Cell Calcium. 1995 Jan;17(1):32-40. doi: 10.1016/0143-4160(95)90100-0.