PMID- 7554451
OWN - NLM
STAT- MEDLINE
DCOM- 19951027
LR  - 20190821
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 76
IP  - 3 Pt 1
DP  - 1995 Sep
TI  - Effects of calorie restriction on transforming growth factor beta 1 and 
      proinflammatory cytokines in murine Sjogren's syndrome.
PG  - 291-6
AB  - The present study was carried out to determine whether restricting dietary 
      calories prevents salivary gland abnormalities and modulates expression of 
      transforming growth factor beta and proinflammatory cytokines, IL-6, and TNF 
      alpha in major salivary glands (SG) of autoimmune lupus-prone (NZB x NZW)F1 (B/W) 
      female mice. These mice develop focal lymphocytic interstitial and periductal 
      round cell infiltrates in salivary glands similar to those of humans with 
      Sjogren's syndrome. Weanling B/W mice were fed a nutritionally adequate 
      semipurified diet either ad libitum (AL) or a calorie-restricted (CR; 40% less 
      calories than AL) diet. The mice were sacrificed at 3.5 months (young) and 8.5 
      months (old) of age. Histopathologic and histomorphometric analyses as well as 
      growth factor and cytokine protein and mRNA expression were carried out in the 
      SG. Histomorphometric analysis of SG from young mice showed no differences 
      between AL and CR mice, but old AL (vs old CR) had a 7.3-fold higher focus score 
      and a 34-fold increase in percentage area inflammation. mRNA analysis revealed 
      significantly higher levels of TGF beta 1 in SG of old CR (6.8-fold) mice. In 
      contrast, CR reduced mRNA expression of proinflammatory cytokines (IL-6, 2.9-fold 
      for young and 4.8-fold for old; TNF alpha, old 3.9-fold). By immunoblotting, 
      significantly higher levels of TGF beta 1 protein was detected in old CR mice (vs 
      old AL; 13.2-fold). IL-6 and TNF alpha proteins were undetectable in both young 
      and old CR groups, whereas an increase in IL-6 (4.7-fold) and TNF alpha 
      (9.3-fold) was observed in old AL mice. These results indicate that amelioration 
      of the histological severity of disease in SG of B/W mice is paralleled and 
      possibly mediated by increased expression of immunosuppressive TGF beta 1 and 
      decreased expression of proinflammatory cytokines.
FAU - Chandrasekar, B
AU  - Chandrasekar B
AD  - Department of Medicine, University of Texas Health Science Center, San Antonio 
      78284-7874, USA.
FAU - McGuff, H S
AU  - McGuff HS
FAU - Aufdermorte, T B
AU  - Aufdermorte TB
FAU - Troyer, D A
AU  - Troyer DA
FAU - Talal, N
AU  - Talal N
FAU - Fernandes, G
AU  - Fernandes G
LA  - eng
GR  - DE-10863/DE/NIDCR NIH HHS/United States
GR  - R01 AG-03417/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cytokines/*biosynthesis/genetics
MH  - Energy Intake/*physiology
MH  - Female
MH  - Interleukin-6/biosynthesis/genetics
MH  - Leukocytes, Mononuclear/physiology
MH  - Mice
MH  - Mice, Inbred NZB
MH  - RNA, Messenger/analysis
MH  - Salivary Glands/cytology
MH  - Sjogren's Syndrome/*immunology
MH  - Transforming Growth Factor beta/*biosynthesis/genetics
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - S0090122985711282 [pii]
AID - 10.1006/clin.1995.1128 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1995 Sep;76(3 Pt 1):291-6. doi: 
      10.1006/clin.1995.1128.