PMID- 7558279
OWN - NLM
STAT- MEDLINE
DCOM- 19951030
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 63
IP  - 10
DP  - 1995 Oct
TI  - Differential antibody isotype reactivity to specific antigens in human lymphatic 
      filariasis: gp15/400 preferentially induces immunoglobulin E (IgE), IgG4, and 
      IgG2.
PG  - 3772-9
AB  - Lymphatic filarial infection in humans is associated with a strong skewing of the 
      immune response towards the TH2 arm, with prominent interleukin 4-producing cells 
      and elevated levels of immunoglobulin G4 (IgG4) and IgE antibodies in peripheral 
      blood. To determine how such a generalized TH2 imbalance governs responses to 
      individual parasite antigens, the profiles of isotypes of antibodies to two 
      recombinant proteins of Brugia spp. were studied. One molecule was the C-terminal 
      portion of the filarial heat shock protein 70 (Bpa-26), representative of a 
      cytoplasmic protein, and the second antigen was a single unit of the tandem 
      repeats of a Brugia polypeptide (BpL-4), a secreted product which is prominently 
      exposed to the immune system. Serum samples from 146 individuals resident in 
      areas in which brugian filariasis is endemic were used, and it was found that 
      whereas the levels of IgG1 and IgG3 responses to both Bpa-26 and BpL-4 were high, 
      IgG4 and IgE antibodies to only BpL-4, not to Bpa-26, were prominent. Thus, an 
      antigen which is chronically exposed to the immune system elicited a 
      TH2-dependent isotype switch, as manifested by increased IgG4 and IgE responses. 
      Moreover, IgG4 and IgE responses to BpL-4 showed a strong negative association, 
      suggesting that mediators other than interleukin 4 must be responsible for such 
      differential regulation of these two isotypes. When the data were analyzed as a 
      function of clinical status, a striking association between elevated levels of 
      IgG3 antibodies to Bpa-26 and manifestation of chronic obstructive disease was 
      found; elephantiasis patients showed significantly higher levels of IgG3 
      antibodies to Bpa-26 than microfilaremics and asymptomatic amicrofilaremics. This 
      indicates that an imbalance of isotypes of antibodies to particular filarial 
      antigens might play a role in the pathogenesis of chronic disease.
FAU - Yazdanbakhsh, M
AU  - Yazdanbakhsh M
AD  - Department of Parasitology, University of Leiden, The Netherlands.
FAU - Paxton, W A
AU  - Paxton WA
FAU - Brandenburg, A
AU  - Brandenburg A
FAU - Van Ree, R
AU  - Van Ree R
FAU - Lens, M
AU  - Lens M
FAU - Partono, F
AU  - Partono F
FAU - Maizels, R M
AU  - Maizels RM
FAU - Selkirk, M E
AU  - Selkirk ME
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Antibodies, Helminth/*biosynthesis
MH  - Antigens, Helminth/*immunology
MH  - Brugia/*immunology
MH  - Elephantiasis, Filarial/*immunology
MH  - Female
MH  - Helminth Proteins/*immunology
MH  - Humans
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis/classification
MH  - Immunoglobulin Isotypes/*biosynthesis
MH  - Male
MH  - Middle Aged
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
PMC - PMC173530
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
PMCR- 1995/10/01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
PHST- 1995/10/01 00:00 [pmc-release]
AID - 10.1128/iai.63.10.3772-3779.1995 [doi]
PST - ppublish
SO  - Infect Immun. 1995 Oct;63(10):3772-9. doi: 10.1128/iai.63.10.3772-3779.1995.