PMID- 7559635 OWN - NLM STAT- MEDLINE DCOM- 19951121 LR - 20210210 IS - 0021-9258 (Print) IS - 0021-9258 (Linking) VI - 270 IP - 42 DP - 1995 Oct 20 TI - Molecular cloning, expression, chromosomal assignment, and tissue-specific expression of a murine alpha-(1,3)-fucosyltransferase locus corresponding to the human ELAM-1 ligand fucosyl transferase. PG - 25047-56 AB - Terminal Fuc alpha 1-3GlcNAc moieties are displayed by mammalian cell surface glycoconjugates in a tissue-specific manner. These oligosaccharides participate in selectin-dependent leukocyte adhesion and have been implicated in adhesive events during murine embryogenesis. Other functions for these molecules remain to be defined, as do the tissue-specific expression patterns of the corresponding alpha-(1-3)-fucosyltransferase (alpha 1-3FT) genes. This report characterizes a murine alpha 1-3FT that shares 77% amino acid sequence identity with human ELAM ligand fucosyltransferase (ELFT, also termed Fuc-TIV). The corresponding gene maps to mouse chromosome 9 in a region of homology with the Fuc-TIV locus on human chromosome 11q. In vitro, the murine alpha 1-3FT can efficiently fucosylate the trisaccharide Gal alpha 1-3Gal beta 1-4GlcNAc (apparent Km of 0.71 mM) to form an unusual tetrasaccharide (Gal alpha 1-3Gal beta 1-4[Fuc alpha 1-3]GlcNAc) described in periimplantation mouse tissues. The enzyme can also form the Lewis x determinant from Gal beta 1-4GlcNAc (Km = 2.05 mM), and the sialyl Lewis x determinant from NeuNAc alpha 2-3Gal beta 1-4GlcNAc (Km = 1.78mM). However, it does not yield sialyl Lewis x determinants when expressed in a mammalian cell line that maintains sialyl Lewis x precursors. Transcripts from this gene accumulate to low levels in hematopoietic organs, but are unexpectedly abundant in epithelia that line the stomach, small intestine, colon, and epididymus. Epithelial cell-specific expression of this gene suggests function(s) in addition to, and distinct from, its proposed role in selectin ligand synthesis. FAU - Gersten, K M AU - Gersten KM AD - Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109-0650, USA. FAU - Natsuka, S AU - Natsuka S FAU - Trinchera, M AU - Trinchera M FAU - Petryniak, B AU - Petryniak B FAU - Kelly, R J AU - Kelly RJ FAU - Hiraiwa, N AU - Hiraiwa N FAU - Jenkins, N A AU - Jenkins NA FAU - Gilbert, D J AU - Gilbert DJ FAU - Copeland, N G AU - Copeland NG FAU - Lowe, J B AU - Lowe JB LA - eng SI - GENBANK/U33457 SI - GENBANK/U33458 GR - 1R01GM47455/GM/NIGMS NIH HHS/United States GR - M01RR00042/RR/NCRR NIH HHS/United States GR - N01-CO-74101/CO/NCI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R RN - 0 (E-Selectin) RN - 0 (Ligands) RN - 0 (RNA, Messenger) RN - EC 2.4.1.- (Fucosyltransferases) RN - EC 2.4.1.152 (galactoside 3-fucosyltransferase) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - *Chromosome Mapping MH - Cloning, Molecular MH - E-Selectin/*metabolism MH - Female MH - Fucosyltransferases/*genetics/metabolism MH - Humans MH - Ligands MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Molecular Sequence Data MH - Organ Specificity MH - RNA, Messenger/analysis EDAT- 1995/10/20 00:00 MHDA- 1995/10/20 00:01 CRDT- 1995/10/20 00:00 PHST- 1995/10/20 00:00 [pubmed] PHST- 1995/10/20 00:01 [medline] PHST- 1995/10/20 00:00 [entrez] AID - S0021-9258(18)87087-9 [pii] AID - 10.1074/jbc.270.42.25047 [doi] PST - ppublish SO - J Biol Chem. 1995 Oct 20;270(42):25047-56. doi: 10.1074/jbc.270.42.25047.