PMID- 7559635
OWN - NLM
STAT- MEDLINE
DCOM- 19951121
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 42
DP  - 1995 Oct 20
TI  - Molecular cloning, expression, chromosomal assignment, and tissue-specific 
      expression of a murine alpha-(1,3)-fucosyltransferase locus corresponding to the 
      human ELAM-1 ligand fucosyl transferase.
PG  - 25047-56
AB  - Terminal Fuc alpha 1-3GlcNAc moieties are displayed by mammalian cell surface 
      glycoconjugates in a tissue-specific manner. These oligosaccharides participate 
      in selectin-dependent leukocyte adhesion and have been implicated in adhesive 
      events during murine embryogenesis. Other functions for these molecules remain to 
      be defined, as do the tissue-specific expression patterns of the corresponding 
      alpha-(1-3)-fucosyltransferase (alpha 1-3FT) genes. This report characterizes a 
      murine alpha 1-3FT that shares 77% amino acid sequence identity with human ELAM 
      ligand fucosyltransferase (ELFT, also termed Fuc-TIV). The corresponding gene 
      maps to mouse chromosome 9 in a region of homology with the Fuc-TIV locus on 
      human chromosome 11q. In vitro, the murine alpha 1-3FT can efficiently fucosylate 
      the trisaccharide Gal alpha 1-3Gal beta 1-4GlcNAc (apparent Km of 0.71 mM) to 
      form an unusual tetrasaccharide (Gal alpha 1-3Gal beta 1-4[Fuc alpha 1-3]GlcNAc) 
      described in periimplantation mouse tissues. The enzyme can also form the Lewis x 
      determinant from Gal beta 1-4GlcNAc (Km = 2.05 mM), and the sialyl Lewis x 
      determinant from NeuNAc alpha 2-3Gal beta 1-4GlcNAc (Km = 1.78mM). However, it 
      does not yield sialyl Lewis x determinants when expressed in a mammalian cell 
      line that maintains sialyl Lewis x precursors. Transcripts from this gene 
      accumulate to low levels in hematopoietic organs, but are unexpectedly abundant 
      in epithelia that line the stomach, small intestine, colon, and epididymus. 
      Epithelial cell-specific expression of this gene suggests function(s) in addition 
      to, and distinct from, its proposed role in selectin ligand synthesis.
FAU - Gersten, K M
AU  - Gersten KM
AD  - Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 
      48109-0650, USA.
FAU - Natsuka, S
AU  - Natsuka S
FAU - Trinchera, M
AU  - Trinchera M
FAU - Petryniak, B
AU  - Petryniak B
FAU - Kelly, R J
AU  - Kelly RJ
FAU - Hiraiwa, N
AU  - Hiraiwa N
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Gilbert, D J
AU  - Gilbert DJ
FAU - Copeland, N G
AU  - Copeland NG
FAU - Lowe, J B
AU  - Lowe JB
LA  - eng
SI  - GENBANK/U33457
SI  - GENBANK/U33458
GR  - 1R01GM47455/GM/NIGMS NIH HHS/United States
GR  - M01RR00042/RR/NCRR NIH HHS/United States
GR  - N01-CO-74101/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (E-Selectin)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.152 (galactoside 3-fucosyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - E-Selectin/*metabolism
MH  - Female
MH  - Fucosyltransferases/*genetics/metabolism
MH  - Humans
MH  - Ligands
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - RNA, Messenger/analysis
EDAT- 1995/10/20 00:00
MHDA- 1995/10/20 00:01
CRDT- 1995/10/20 00:00
PHST- 1995/10/20 00:00 [pubmed]
PHST- 1995/10/20 00:01 [medline]
PHST- 1995/10/20 00:00 [entrez]
AID - S0021-9258(18)87087-9 [pii]
AID - 10.1074/jbc.270.42.25047 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Oct 20;270(42):25047-56. doi: 10.1074/jbc.270.42.25047.