PMID- 7561060
OWN - NLM
STAT- MEDLINE
DCOM- 19951027
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 155
IP  - 7
DP  - 1995 Oct 1
TI  - A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the 
      transendothelial migration of monocytes in vitro.
PG  - 3610-8
AB  - Chemokines secreted by endothelium may promote diapedesis of leukocytes by a 
      gradient-dependent chemotactic mechanism or by stimulating random motility so 
      that leukocytes transmigrate in a gradient-independent manner. Alternatively, 
      chemokines may bind to endothelium and extracellular matrix to stimulate 
      haptotactic migration. We first analyzed the role of the chemokine monocyte 
      chemoattractant protein-1 (MCP-1) in the migration of human monocytes across 
      untreated or IL-1-stimulated HUVEC monolayers cultured on human amnion. Then we 
      further examined whether MCP-1-dependent transmigration occurred through a 
      chemokinetic, chemotactic, or haptotactic mechanism. A neutralizing mAb against 
      MCP-1 inhibited passage of monocytes across untreated or IL-1-stimulated HUVEC by 
      74 +/- 3% and 45 +/- 4%, respectively. Addition of MCP-1 itself to the apical 
      compartment of unstimulated HUVEC/amnion cultures also reduced the transmigration 
      of monocytes, in this instance by 73 +/- 9%. MCP-1 suppressed diapedesis only 
      when present above the endothelium at a concentration equal to or greater than 
      that endogenously deposited beneath the endothelium, and its inhibitory action 
      could be overcome by addition of more concentrated MCP-1 below the HUVEC 
      cultures. As much as 90% of the MCP-1 secreted into the underlying basement 
      membrane and connective tissue could be washed out of HUVEC/amnion cultures; this 
      procedure decreased transmigration by 69 +/- 4%. These data indicate that MCP-1 
      promotes transmigration of monocytes, but only when present in a gradient across 
      endothelial monolayers. They further suggest that this gradient is predominantly 
      soluble, rather than haptotactic.
FAU - Randolph, G J
AU  - Randolph GJ
AD  - Department of Pathology, School of Medicine, State University of New York at 
      Stony Brook 11794, USA.
FAU - Furie, M B
AU  - Furie MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism/*pharmacology
MH  - Culture Media, Conditioned
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Humans
MH  - Monocytes/*cytology/metabolism
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Oct 1;155(7):3610-8.