PMID- 7561346
OWN - NLM
STAT- MEDLINE
DCOM- 19951108
LR  - 20110727
IS  - 0021-5325 (Print)
IS  - 0021-5325 (Linking)
VI  - 69
IP  - 7
DP  - 1995 Jul
TI  - [Affinity of estrogen binding in the cultured spinal ligament cells: an in vitro 
      study using cells from spinal ligament ossification patients].
PG  - 440-9
AB  - This immunological study investigated the effects of estrogen as a potential 
      causative factor for spinal ligament ossification (such as ossification of the 
      posterior longitudinal ligament, OPLL; ossification of the yellow ligament, OYL; 
      and ossification of the anterior longitudinal ligament, OALL). The serum total 
      estrogen (estron + estradiol + estriol) level in the OPLL patients and controls 
      was measured by radioimmunoassay. To determine any difference in the affinity of 
      the estrogen, 3,17 beta-estradiol receptors of cultured spinal ligament cells 
      obtained from OPLL patient were detected by receptor binding assay, and compared 
      with cells from controls. Additionally, to evaluate the responses of cultured 
      spinal ligament cells to stimulation by 3,17 beta-estradiol, examined the 
      production of bone Gla protein (BGP) in medium, the rate of tritiated thymidine 
      (3H-TdR) uptake, and change in affinity of transforming growth factor-beta 1 
      (TGF-beta 1) receptor on those cells with 3,17 beta-estradiol added (E2+ group), 
      and compared the results with those when 3,17 beta-estradiol was not added (E2- 
      group). The serum total estrogen level was significantly higher in OPLL patients 
      than in controls, and the level increased with increasing extent of ligament 
      ossification. Cultured cells obtained from OPLL patients had receptors with a 
      higher affinity for 3,17 beta-estradiol than did cells from controls. Cells 
      obtained from OPLL patients responded to the stimulation by 3,17 beta-estradiol, 
      accelerated BGP production, and elevated the 3H-TdR uptake. However, cells from 
      controls showed no change in the stimulation by 3,17 beta-estradiol.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Wada, A
AU  - Wada A
AD  - Department of Orthopedic Surgery, Toho University School of Medicine, Tokyo, 
      Japan.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Nihon Seikeigeka Gakkai Zasshi
JT  - Nihon Seikeigeka Gakkai zasshi
JID - 0413716
RN  - 0 (Estrogens)
RN  - 0 (Receptors, Estradiol)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cells, Cultured
MH  - Estrogens/*metabolism
MH  - Female
MH  - Humans
MH  - Ligaments/cytology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Ossification of Posterior Longitudinal Ligament/etiology/*metabolism
MH  - Receptors, Estradiol/metabolism
MH  - *Spine
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Seikeigeka Gakkai Zasshi. 1995 Jul;69(7):440-9.