PMID- 7561523
OWN - NLM
STAT- MEDLINE
DCOM- 19951108
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 58
IP  - 4
DP  - 1995 Oct
TI  - Monomeric human IgE evokes a transient calcium rise in individual human 
      neutrophils.
PG  - 459-67
AB  - Digital fluorescence calcium imaging was used to investigate and identify the 
      primary biological responses of human neutrophils to monomeric immunoglobulin E 
      (IgE). Treatment of neutrophils with IgE caused a transient rise in the level of 
      intracellular calcium that was inhibited by pertussis toxin. The calcium rise was 
      due mainly to release from an intracellular membrane-enclosed store that is also 
      sensitive to the chemotactic peptide formyl-Met-Leu-Phe. The IgE-induced calcium 
      transient was independent of Fc gamma receptors and of Fc epsilon receptor 
      ligation. Our data suggest that the mere binding of IgE to neutrophils is 
      sufficient to evoke a biological response without the need for IgE/receptor 
      cross-linking.
FAU - Collison, K S
AU  - Collison KS
AD  - Biological and Medical Research Department, King Faisal Specialist Hospital and 
      Research Centre, Riyadh, Saudi Arabia.
FAU - Kwaasi, A A
AU  - Kwaasi AA
FAU - Parhar, R S
AU  - Parhar RS
FAU - al-Sedairy, S T
AU  - al-Sedairy ST
FAU - al-Mohanna, F A
AU  - al-Mohanna FA
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Receptors, IgG)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Calcium/*blood
MH  - Extracellular Space/chemistry/drug effects/metabolism
MH  - Fluorescence
MH  - Homeostasis/drug effects
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunoglobulin E/*pharmacology
MH  - Intracellular Fluid/chemistry/drug effects/metabolism
MH  - Molecular Sequence Data
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/*metabolism/ultrastructure
MH  - Receptors, IgG/physiology
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/jlb.58.4.459 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1995 Oct;58(4):459-67. doi: 10.1002/jlb.58.4.459.