PMID- 7561782
OWN - NLM
STAT- MEDLINE
DCOM- 19951113
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 76 ( Pt 9)
DP  - 1995 Sep
TI  - A glycoprotein E deletion mutant of bovine herpesvirus 1 infects the same limited 
      number of tissues in calves as wild-type virus, but for a shorter period.
PG  - 2387-92
AB  - To gain insight into the role of glycoprotein E of bovine herpesvirus 1 (BHV-1), 
      we compared the distribution of wild-type (wt) BHV-1 with that of a gE deletion 
      mutant (gE-) in calves after intranasal inoculation. The wt-infected calves had 
      severe clinical signs, but the gE(-)-infected calves were virtually free of 
      clinical signs. At 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one 
      calf from each group was killed and tissues were collected for virus isolation 
      and PCR analysis. At 3, 4, 7 and 8 days p.i., infectious virus could be isolated 
      only from the nasopharyngeal mucosa, parotid gland and nearby lymphoid tissues 
      for both the wt- and gE(-)-infected calves. At 3 and 4 days p.i., virus titres in 
      these tissues were comparable in both the wt- and gE(-)-infected calves. However, 
      the virus titres were significantly reduced at 7 and 8 days p.i. in the 
      gE(-)-infected calves, but not in the wt-infected calves. Semi-quantitative PCR 
      analysis revealed that for the entire infection period (3 to 51 days p.i.) 
      significantly more BHV-1 DNA was detected in the trigeminal ganglia (TG) of the 
      wt-infected calves than in those of the gE(-)-infected calves. We conclude that 
      the gE- mutant infects the same limited number of tissues as wt BHV-1, but for a 
      shorter period.
FAU - van Engelenburg, F A
AU  - van Engelenburg FA
AD  - Open Department of Virology, Institute for Animal Science and Health, Lelystad, 
      The Netherlands.
FAU - Kaashoek, M J
AU  - Kaashoek MJ
FAU - van Oirschot, J T
AU  - van Oirschot JT
FAU - Rijsewijk, F A
AU  - Rijsewijk FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (bovine herpesvirus type-1 glycoproteins)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*virology
MH  - DNA, Viral/analysis
MH  - Gene Deletion
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Herpesvirus 1, Bovine/*genetics/pathogenicity
MH  - Laryngeal Mucosa/virology
MH  - Lymphoid Tissue/virology
MH  - Parotid Gland/virology
MH  - Time Factors
MH  - Trigeminal Ganglion/virology
MH  - Viral Envelope Proteins/*genetics
MH  - Viral Proteins
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1099/0022-1317-76-9-2387 [doi]
PST - ppublish
SO  - J Gen Virol. 1995 Sep;76 ( Pt 9):2387-92. doi: 10.1099/0022-1317-76-9-2387.