PMID- 7561787
OWN - NLM
STAT- MEDLINE
DCOM- 19951103
LR  - 20190830
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 46
IP  - 3
DP  - 1995 Jul
TI  - Targeted integration of human herpesvirus 6 in the p arm of chromosome 17 of 
      human peripheral blood mononuclear cells in vivo.
PG  - 178-88
AB  - Out of 64 cases of non-Hodgkin's lymphomas (NHL), 55 cases of Hodgkin's disease 
      (HD) and 31 cases of multiple sclerosis (MS), 2 NHL, 7 HD and 1 MS cases were 
      found positive by polymerase chain reaction (PCR) for the presence of HHV-6 
      sequences in pathologic lymph nodes of the lymphomas and in peripheral blood 
      mononuclear cells (PBMCs) of MS. A further analysis of the PBMCs of the PCR 
      positive cases by standard Southern blot technique revealed only 2 NHL, 3 HD and 
      1 MS cases as positive, indicating that these six patients have an unusually high 
      viral copy number in the PBMCs. Restriction analysis, carried out using probes 
      representative of different regions of the virus, showed that three cases retain 
      only a deleted portion of the viral genome. In the remaining three cases a 
      complete viral genome was present, containing the right end sequences in which 
      the rep-like gene, possibly crucial to the viral and cellular life cycle, is 
      located. The analysis by pulsed field gel electrophoresis (PFGE) of the total DNA 
      of the PBMCs obtained directly, without culture from PBMCs of these last three 
      cases (1 NHL, 1 HD, and 1 MS), using the same probes, showed the absence of free 
      viral molecules and the association of viral sequences with high molecular weight 
      DNA. These results are consistent with in vivo integration of the entire virus in 
      the cellular genome. A further study of the same patients with chromosome 
      fluorescence in situ hybridization (FISH) showed in all the three cases the 
      presence of a specific hybridization site, located at the telomeric extremity of 
      the short arm of chromosome 17 (17p13), suggesting that this location is at least 
      a preferred site of an infrequent, but possibly biologically important, 
      integration phenomenon.
FAU - Torelli, G
AU  - Torelli G
AD  - Department of Medical Sciences, University of Modena, Italy.
FAU - Barozzi, P
AU  - Barozzi P
FAU - Marasca, R
AU  - Marasca R
FAU - Cocconcelli, P
AU  - Cocconcelli P
FAU - Merelli, E
AU  - Merelli E
FAU - Ceccherini-Nelli, L
AU  - Ceccherini-Nelli L
FAU - Ferrari, S
AU  - Ferrari S
FAU - Luppi, M
AU  - Luppi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Antibodies, Viral/blood
MH  - Blotting, Southern
MH  - Cell Line
MH  - *Chromosomes, Human, Pair 17
MH  - DNA, Viral/analysis
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Female
MH  - Herpesviridae Infections/genetics/immunology/*virology
MH  - Herpesvirus 6, Human/*genetics/immunology
MH  - Hodgkin Disease/virology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocytes, Mononuclear/*virology
MH  - Lymph Nodes/virology
MH  - Lymphoma, Non-Hodgkin/virology
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/virology
MH  - Polymerase Chain Reaction
MH  - *Virus Integration
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1002/jmv.1890460303 [doi]
PST - ppublish
SO  - J Med Virol. 1995 Jul;46(3):178-88. doi: 10.1002/jmv.1890460303.