PMID- 7567454
OWN - NLM
STAT- MEDLINE
DCOM- 19951106
LR  - 20190524
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 23
IP  - 17
DP  - 1995 Sep 11
TI  - Transcriptional regulation of the Sex-lethal gene by helix-loop-helix proteins.
PG  - 3441-8
AB  - Somatic sex determination in Drosophila depends on the expression of Sex-lethal 
      (Sxl), whose level is determined by the relative number of X chromosomes and sets 
      of autosomes (X:A ratio). The first step in regulation of Sxl expression is 
      transcriptional control from its early promoter and several genes encoding 
      transcription factors of the helix-loop-helix (HLH) family such as daughterless 
      (da), sisterless-b (sis-b), deadpan (dpn) and extramacrochaetae (emc) have been 
      implicated. By the use of transfection assays and in vitro binding experiments, 
      here we show that da/sis-b heterodimers bind several sites on the Sxl early 
      promoter with different affinities and consequently tune the level of active 
      transcription from this promoter. Interestingly, our data indicate that 
      repression by the dpn product of da/sis-b dependent activation results from 
      specific binding of dpn protein to a unique site within the promoter. This 
      contrasts with the mode of emc repression, which inhibits the formation of the 
      da/sis-b heterodimers. These results reveal the molecular mechanisms by which Sxl 
      gene transcription is positively or negatively regulated to control somatic sex 
      determination.
FAU - Hoshijima, K
AU  - Hoshijima K
AD  - Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FAU - Kohyama, A
AU  - Kohyama A
FAU - Watakabe, I
AU  - Watakabe I
FAU - Inoue, K
AU  - Inoue K
FAU - Sakamoto, H
AU  - Sakamoto H
FAU - Shimura, Y
AU  - Shimura Y
LA  - eng
SI  - GENBANK/D50435
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Da protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Hormones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Sxl protein, Drosophila)
RN  - 0 (Transcription Factors)
RN  - 0 (sc protein, Drosophila)
RN  - 149347-68-2 (Dpn protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - DNA-Binding Proteins/genetics/metabolism/*physiology
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/*genetics
MH  - *Gene Expression Regulation
MH  - *Helix-Loop-Helix Motifs
MH  - Insect Hormones/*genetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism
MH  - Oligodeoxyribonucleotides/chemistry
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - *RNA-Binding Proteins
MH  - Repressor Proteins/*chemistry
MH  - *Sex Determination Analysis
MH  - Transcription Factors/metabolism/*physiology
PMC - PMC307222
EDAT- 1995/09/11 00:00
MHDA- 1995/09/11 00:01
CRDT- 1995/09/11 00:00
PHST- 1995/09/11 00:00 [pubmed]
PHST- 1995/09/11 00:01 [medline]
PHST- 1995/09/11 00:00 [entrez]
AID - 5c0099 [pii]
AID - 10.1093/nar/23.17.3441 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1995 Sep 11;23(17):3441-8. doi: 10.1093/nar/23.17.3441.