PMID- 7570873
OWN - NLM
STAT- MEDLINE
DCOM- 19951103
LR  - 20190512
IS  - 0035-9203 (Print)
IS  - 0035-9203 (Linking)
VI  - 89
IP  - 4
DP  - 1995 Jul-Aug
TI  - Isolation of Trypanosoma brucei gambiense from northern Uganda: evaluation of the 
      kit for in vitro isolation (KIVI) in an epidemic focus.
PG  - 388-9
AB  - 867 individuals from 3 sites near the town of Adjumani in the East Moyo region of 
      north-west Uganda were investigated clinically and serologically for evidence of 
      current trypanosome infections. Blood samples were taken from 94 persons with a 
      positive card agglutination test for trypanosomiasis (CATT) and clinical suspects 
      and inoculated into the kit for in vitro isolation of Trypanosoma brucei 
      gambiense (KIVI). Amongst this group, 30 parasitaemic individuals were identified 
      by microhaematocrit centrifugation and the quantitative buffy coat technique 
      (QBC). Only 80% of these isolates, and one isolate from an aparasitaemic 
      individual, grew in culture. The success or failure of cultures from parasitaemic 
      patients was unrelated to the size of the trypanosome inoculum. The implications 
      of these results and possible reasons for the failure of KIVI are discussed.
FAU - McNamara, J J
AU  - McNamara JJ
AD  - MRC Trypanosomiasis Research Group, University of Bristol, Langford, UK.
FAU - Bailey, J W
AU  - Bailey JW
FAU - Smith, D H
AU  - Smith DH
FAU - Wakhooli, S
AU  - Wakhooli S
FAU - Godfrey, D G
AU  - Godfrey DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Trans R Soc Trop Med Hyg
JT  - Transactions of the Royal Society of Tropical Medicine and Hygiene
JID - 7506129
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Animals
MH  - Disease Outbreaks
MH  - Humans
MH  - *Reagent Kits, Diagnostic
MH  - Trypanosoma brucei gambiense/*isolation & purification
MH  - Trypanosomiasis, African/*diagnosis/epidemiology/parasitology
MH  - Uganda/epidemiology
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1016/0035-9203(95)90021-7 [doi]
PST - ppublish
SO  - Trans R Soc Trop Med Hyg. 1995 Jul-Aug;89(4):388-9. doi: 
      10.1016/0035-9203(95)90021-7.