PMID- 7571948
OWN - NLM
STAT- MEDLINE
DCOM- 19951026
LR  - 20061115
IS  - 0001-5334 (Print)
IS  - 0001-5334 (Linking)
VI  - 28
IP  - 2
DP  - 1995 Jun
TI  - [Membrane fusion between Ehrlich ascites of mastocarcinoma cells and liposome 
      induced by proton translocation of transplasma membrane NADH-ferricyanide redox 
      enzymes].
PG  - 137-45
AB  - By using resonance energy transfer assay (RET) and fluorescence microscopy we 
      show experimental evidence that membrane fusion of Ehrlich ascites of 
      mastocarcinoma cells with liposomes could be induced by the proton translocation 
      activity associated with NADH-ferricyanide redox enzyme of transplasma membrane 
      of cancer cells. The iodoacetate, an inhibitor of glycolysis, was found to be 
      able to depress the proton translocation activity and also to inhibit the 
      membrane fusion. It is suggested that NADH produced mainly by glycolysis is 
      utilized as the substrate (electron donor) for transmembrane ferricyanide 
      reduction, and the proton pumping activity in the cancer cells is coupled to the 
      transmembrane NADH-ferricyanide redox enzyme system. Experiments also show that 
      membrane fusion extent of cancer cells with liposomes is proportional to the 
      amount of H+ pumped out by the cells and membrane fusion process also exhibits a 
      H+ consuming mode just as in fusion process of mitochondria with liposomes by 
      redox enzyme proton pumps of respiratory chain. All of the results presented in 
      this paper consists with recent reports of this laboratory, which indicated that 
      various types of proton pumping system from different membrane system of cell 
      have a new function in membrane fusion. Therefore, the proton pumping induced 
      membrane fusion may have a more general physiological importance in triggering 
      and modulating fusion process of native membrane in vivo.
FAU - Liu, S S
AU  - Liu SS
AD  - State Key Laboratory of Membrane & Membrane Biotechnology, Institute of Zoology, 
      Academia Sinica, Beijing.
FAU - Wu, J H
AU  - Wu JH
FAU - Li, Q H
AU  - Li QH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Shi Yan Sheng Wu Xue Bao
JT  - Shi yan sheng wu xue bao
JID - 0413570
RN  - 0 (Liposomes)
RN  - 0 (Proton Pumps)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 1.6.99.- (ferricyanide reductase)
SB  - IM
MH  - Animals
MH  - Carcinoma, Ehrlich Tumor/metabolism/*pathology
MH  - Glycolysis
MH  - Liposomes
MH  - Membrane Fusion/*physiology
MH  - Mice
MH  - NADH, NADPH Oxidoreductases/physiology
MH  - Oxidation-Reduction
MH  - Proton Pumps/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
PST - ppublish
SO  - Shi Yan Sheng Wu Xue Bao. 1995 Jun;28(2):137-45.