PMID- 75724
OWN - NLM
STAT- MEDLINE
DCOM- 19780417
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 56
IP  - 1-2
DP  - 1978
TI  - Antigenic relatedness of equine herpes virus types 1 and 3.
PG  - 33-45
AB  - Antiserums prepared in specific pathogen free (SPF) ponies were used in direct 
      and indirect immunofluorescence, immunodiffusion, complement fixation and serum 
      neutralization procedures to study the interrelationships of the three types of 
      equine herpes viruses (EHV-1, EHV-2, and EHV-3). Equine cell cultures infected 
      with each type virus fluoresced when stained with homologous conjugated 
      antiserum. In reciprocal tests EHV-1 and EHV-3 cross-fluoresced, but EHV-2 did 
      not cross-fluoresce. Non-infected cell cultures did not fluoresce when stained 
      with the 3 conjugates. EHV-1 and EHV-3 cross-fluoresced in reciprocal indirect 
      fluorescent antibody tests, but no cross-fluorescence was shown with EHV-2. 
      Antigens representing each type of equine herpes virus reacted with their 
      homologous antiserum in the immunodiffusion test. In reciprocal tests, a common 
      line(s) of identity formed with EHV-1 and EHV-3; however, the precipitin line(s) 
      was not common with EHV-2. Antigen prepared from noninfected embryonic mule skin 
      (EMS) cell cultures did not react with any of the antiserums. Specific 
      complement-fixing antibodies were present in antiserums when tested against their 
      homologous antigens. In reciprocal complement fixation tests EHV-1 and EHV-3 
      crossreacted, but no cross-reactivity was shown with EHV-2. Significant levels of 
      neutralizing antibody were in an antiserum when tested against homologous virus, 
      whereas cross-neutralization was not detectable in reciprocal tests. These 
      studies indicate that each type of equine herpes virus contains specific 
      antigenic components, and EHV-1 and EHV-3 share a common antigen(s) that is not 
      shared with EHV-2.
FAU - Gutekunst, D E
AU  - Gutekunst DE
FAU - Malmquist, W A
AU  - Malmquist WA
FAU - Becvar, C S
AU  - Becvar CS
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Antigens, Viral/*analysis
MH  - Complement Fixation Tests
MH  - Cross Reactions
MH  - Epitopes
MH  - Fluorescent Antibody Technique
MH  - Herpesviridae/*immunology
MH  - Herpesvirus 1, Equid/*immunology
MH  - Horses
MH  - Immunodiffusion
MH  - Neutralization Tests
EDAT- 1978/01/01 00:00
MHDA- 1978/01/01 00:01
CRDT- 1978/01/01 00:00
PHST- 1978/01/01 00:00 [pubmed]
PHST- 1978/01/01 00:01 [medline]
PHST- 1978/01/01 00:00 [entrez]
AID - 10.1007/BF01317281 [doi]
PST - ppublish
SO  - Arch Virol. 1978;56(1-2):33-45. doi: 10.1007/BF01317281.