PMID- 7574153
OWN - NLM
STAT- MEDLINE
DCOM- 19951109
LR  - 20031114
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 56
IP  - 7
DP  - 1995 Jul
TI  - Abortion in heifers inoculated with a thymidine kinase-negative recombinant of 
      bovine herpesvirus 1.
PG  - 870-4
AB  - The Copper isolate of bovine herpesvirus 1 (BHV-1) was used to produce a 
      thymidine kinase-negative (TK-) recombinant by insertion of a beta-galactosidase 
      (bgal) expression cassette into the TK coding region. The recombinant virus (TK- 
      bgal+) was tested for abortifacient activity in cattle by inoculation of 5 
      pregnant heifers at 25 to 29 weeks gestation. Five additional heifers were 
      inoculated with the Cooper TK-positive (TK+) virus to serve as controls. After 
      inoculation, both groups of heifers developed similar febrile responses and 
      neutralizing antibody titers. Virus was isolated from blood of all heifers during 
      the first postinoculation (PI) week, and isolation frequencies were similar for 
      both groups. In contrast, whereas virus was isolated from many of the nasal and 
      vaginal swab specimens of heifers inoculated with TK+ virus, only rare virus 
      isolations were made from the heifers given TK- bgal+ virus. All heifers 
      inoculated with TK+ virus aborted between PI days 19 and 35. The finding of 
      characteristic microscopic lesions and viral antigen in fetal tissues indicated 
      that the abortions were caused by BHV-1 infection. Virus was isolated from 3 
      fetuses, and all isolates were TK+ virus. Two heifers inoculated with TK- bgal+ 
      virus aborted at PI days 25 and 39. Fetal tissues had typical BHV-1 microscopic 
      lesions and viral antigen. Virus was isolated from blood of both fetuses, and the 
      isolates were TK- bgal+. Results of this study indicate that inactivation of the 
      TK gene reduces, but does not eliminate, the abortifacient activity of BHV-1.
FAU - Miller, J M
AU  - Miller JM
AD  - USDA, National Animal Disease Center, Ames, IA 50010, USA.
FAU - Whetstone, C A
AU  - Whetstone CA
FAU - Bello, L J
AU  - Bello LJ
FAU - Lawrence, W C
AU  - Lawrence WC
FAU - Whitbeck, J C
AU  - Whitbeck JC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Abortifacient Agents)
RN  - EC 2.7.1.21 (Thymidine Kinase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - *Abortifacient Agents
MH  - *Abortion, Veterinary
MH  - Animals
MH  - Cattle
MH  - Female
MH  - Fetal Death/veterinary
MH  - Gene Deletion
MH  - *Herpesvirus 1, Bovine/genetics
MH  - Mutagenesis, Insertional
MH  - Pregnancy
MH  - Recombination, Genetic
MH  - Thymidine Kinase
MH  - beta-Galactosidase/biosynthesis
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
PST - ppublish
SO  - Am J Vet Res. 1995 Jul;56(7):870-4.