PMID- 7575568
OWN - NLM
STAT- MEDLINE
DCOM- 19951102
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 214
IP  - 3
DP  - 1995 Sep 25
TI  - Nitric oxide regulates IL-8 expression in melanoma cells at the transcriptional 
      level.
PG  - 949-56
AB  - We investigated the role of nitric oxide (NO) in the expression of interleukin-8 
      (IL-8) in the human melanoma cell line, G361. Three NO donors, 
      3-morpholinosydnonimine hydrochloride (SIN-1), S-nitroso-N-acetylpenicillamine 
      (SNAP), and S-nitroso-L-glutathione (SNOG), all caused an increase in both IL-8 
      protein secretion and promoter activity. Truncation of the promoter showed that 
      101 bp of the 5' flanking region proximal to the transcription start site are 
      sufficient for the response to NO. Furthermore, mutation of the NF-kappa B and 
      NF-IL-6 binding sites led to a significant decrease in NO-stimulated promoter 
      activity. The nitric oxide synthase inhibitor, NG-amino-L-homoarginine (NAHA), 
      inhibited TNF-alpha-stimulated IL-8 promoter activity by 60%. Addition of excess 
      L- but not D-arginine partially reversed the NAHA-mediated inhibition. These 
      results demonstrate that NO is an endogenous regulator of IL-8 production in G361 
      melanoma cells.
FAU - Andrew, P J
AU  - Andrew PJ
AD  - Dept. General Dermatology, Sandoz Forschungsinstitut, Vienna, Austria.
FAU - Harant, H
AU  - Harant H
FAU - Lindley, I J
AU  - Lindley IJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitroso Compounds)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 0 (Transcription Factors)
RN  - 0 (Vasodilator Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 57564-91-7 (S-Nitrosoglutathione)
RN  - 5O5U71P6VQ (linsidomine)
RN  - 79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
RN  - D46583G77X (Molsidomine)
RN  - GAN16C9B8O (Glutathione)
RN  - GNN1DV99GX (Penicillamine)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Proteins
MH  - Cell Line
MH  - DNA Primers
MH  - DNA-Binding Proteins/metabolism
MH  - Gene Expression/*drug effects
MH  - Glutathione/*analogs & derivatives/pharmacology
MH  - Humans
MH  - Interleukin-8/*biosynthesis
MH  - Melanoma
MH  - Molecular Sequence Data
MH  - Molsidomine/*analogs & derivatives/pharmacology
MH  - Mutagenesis, Site-Directed
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/*pharmacology
MH  - Nitroso Compounds/*pharmacology
MH  - Nuclear Proteins/metabolism
MH  - Penicillamine/*analogs & derivatives/pharmacology
MH  - Platelet Aggregation Inhibitors/*pharmacology
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic/*drug effects
MH  - S-Nitroso-N-Acetylpenicillamine
MH  - S-Nitrosoglutathione
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic/*drug effects
MH  - Tumor Cells, Cultured
MH  - Vasodilator Agents/*pharmacology
EDAT- 1995/09/25 00:00
MHDA- 1995/09/25 00:01
CRDT- 1995/09/25 00:00
PHST- 1995/09/25 00:00 [pubmed]
PHST- 1995/09/25 00:01 [medline]
PHST- 1995/09/25 00:00 [entrez]
AID - S0006-291X(85)72378-9 [pii]
AID - 10.1006/bbrc.1995.2378 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Sep 25;214(3):949-56. doi: 
      10.1006/bbrc.1995.2378.